Marek's Disease Herpesvirus-Induced DNA Polymerase
Marek's Disease Herpesvirus-Induced DNA Polymerase
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马立克氏病疱疹病毒诱导的 DNA 聚合酶
DOI：
10.1128/jvi.14.5.1209-1219.1974
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发表时间：
1974
影响因子：
5.4
通讯作者：
H. Towle
中科院分区：
文献类型：
--
作者：
J. Boezi;Lucy F. Lee;R. Blakesley;M. Koenig;H. Towle
用马立克氏病疱疹病毒GA株感染鸭胚胎成纤维细胞，诱导了一种新的DNA聚合酶的产生。这种新的DNA聚合酶被命名为MDHV诱导的DNA聚合酶，它与未感染的鸭胚胎成纤维细胞的DNA聚合酶活性可以通过其对磷酸纤维素的层析行为、沉降系数和催化性质来区分。对磷酸纤维素柱层析纯化的MDHV诱导的DNA聚合酶的性质进行了研究。在0.25M KCl存在下，蔗糖密度梯度离心法测得该酶的沉降系数为5.9s。根据这个沉降系数，MDHV诱导的DNA聚合酶的分子量估计为100,000。MDHV诱导的DNA聚合酶不能有效地用Poly(Da)·寡聚(DT)12-18或Poly(DC)·寡聚(DG)12-18作为模板-引物。未感染鸭胚成纤维细胞的DNA聚合酶可以使用这些合成的模板-引物。MDHV诱导的DNA聚合酶也不能以聚(Ra)·寡聚(Dt)12-18或聚(Rc)·寡聚(Dg)12-18为模板引物，也不能以寡聚(Dt)12-18为引物，表明它不是R-DNA聚合酶、逆转录酶或末端核苷酸转移酶的聚合酶。在体外，(NH4)2SO4对MDHV诱导的DNA聚合酶的合成有明显的抑制作用。
Infection of duck embryo fibroblasts by Marek's disease herpesvirus (MDHV), strain GA, led to the induction of a novel DNA polymerase. This novel DNA polymerase, designated MDHV-induced DNA polymerase, could be distinguished from the DNA polymerase activities of uninfected duck embryo fibroblasts by its chromatographic behavior on phosphocellulose, by its sedimentation coefficient, and by its catalytic properties. The characteristics of MDHV-induced DNA polymerase which had been purified by phosphocellulose chromatography were investigated. The sedimentation coefficient of the enzyme, as determined by sucrose density gradient centrifugation in the presence of 0.25 M KCl, was 5.9S. From this sedimentation coefficient, the molecular weight of MDHV-induced DNA polymerase was estimated to be 100,000. MDHV-induced DNA polymerase could not effectively use either poly(dA)·oligo(dT)12-18 or poly(dC)·oligo(dG)12-18 as a template-primer. The DNA polymerases from uninfected duck embryo fibroblasts could use these synthetic template-primers. MDHV-induced DNA polymerase also could not use poly(rA)·oligo(dT)12-18 or poly(rC)·oligo(dG)12-18 as template-primers or oligo(dT)12-18 as a primer, indicating that it was not a polymerase of the type R-DNA polymerase, reverse transcriptase, or a terminal nucleotidyl transferase. In vitro synthesis of DNA by MDHV-induced DNA polymerase was markedly inhibited by the addition of (NH4)2SO4 to the reaction mixture.