In vitro propagation and inducible differentiation of multipotential progenitor cells from human fetal brain
In vitro propagation and inducible differentiation of multipotential progenitor cells from human fetal brain
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DOI：
10.1016/s0306-4522(96)00386-7
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发表时间：
1997-02-01
期刊：
影响因子：
3.3
通讯作者：
Fine, A
中科院分区：
文献类型：
--
作者：
ChalmersRedman, RME;Priestley, T;Fine, A
中枢神经系统神经元和神经胶质细胞起源于未分化的胚胎神经上皮细胞。当在含有表皮生长因子和胰岛素样生长因子-I的培养基中的非粘附性基质上生长时，来自人胎儿前脑的这种祖细胞可以在体外繁殖延长的时间。这些活跃分裂的细胞可以被诱导分化成各种组织化学特征的神经元和神经胶质细胞与它们的前脑起源一致。电生理记录表明，从这些祖细胞分化的神经元成熟缓慢，并显示一系列的谷氨酸和GABA介导的电导正常哺乳动物前脑neurons.Our观察支持这些营养因子在人类大脑的正常发育中的作用。本文所述的方法可以提供丰富的正常的，未转化的人前脑神经元和神经胶质细胞的研究和治疗应用。版权所有（C）1996 IBRO。出版社：Elsevier Science Ltd
Central nervous system neurons and glia arise from undifferentiated embryonic neuroepithelial cells. Such progenitor cells from the human fetal forebrain can be propagated in vitro for extended periods, when grown on non-adhesive substrates in medium containing epidermal growth factor and insulinlike growth factor-I. These actively-dividing cells can be induced to differentiate into a variety of histochemically-characterized neurons and glia consistent with their forebrain origin. Electrophysiological recording indicates that differentiated neurons derived from these progenitors mature slowly, and display a range of glutamate- and GABA-mediated conductances characteristic of normal mammalian forebrain neurons.Our observations support a role for these trophic factors in normal development of the human brain. The methods described here may provide abundant normal, untransformed human forebrain neurons and glia for research and therapeutic applications. Copyright (C) 1996 IBRO. Published by Elsevier Science Ltd.