Development of 11 polymorphic microsatellite markers for Xylocarpus granatum (Meliaceae) using next-generation sequencing technology.
Development of 11 polymorphic microsatellite markers for Xylocarpus granatum (Meliaceae) using next-generation sequencing technology.
复制标题
使用下一代测序技术开发 Xylocarpus granatum（楝科）的 11 个多态性微卫星标记。
DOI：
10.1007/s12686-013-9990-9
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发表时间：
2013
期刊：
影响因子：
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et al.
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作者：
Tomizawa Y. & Shinmura Y.;et al.
人类的影响已经严重破坏了红树林，红树林的保护将需要有关当地和区域种群遗传结构的信息。在这里，我们报告了11个新的微卫星标记的开发和多态性，这些标记是通过下一代测序在印度-西太平洋地区9个种群的56个广泛分布的红树林物种xylocarpus granatum（Meliaceae）样本中开发的。所有位点均呈多态性，每个位点的等位基因数在4 ~ 19个之间。在马来西亚沙巴的一个种群中，每个位点的平均观察杂合度和预期杂合度分别为0.59和0.58。所有位点均未检测到空等位基因、显著连锁不平衡或偏离Hardy-Weinberg平衡。所开发的11个标记可以作为该物种在其分布范围内的保护遗传学的有价值的工具。
Human impacts have seriously damaged mangroves, and conservation of mangroves will require information on local and regional population genetic structures. Here, we report the development and polymorphism of eleven novel microsatellite markers, developed using next-generation sequencing on 56 samples of widespread mangrove speciesXylocarpus granatum(Meliaceae) from nine populations across the Indo-West Pacific region. All loci were found to be polymorphic, with the number of alleles per locus ranging from four to 19. In a population from Sabah (Malaysia), the mean observed and expected heterozygosity per locus was 0.59 and 0.58, respectively. No null allele, significant linkage disequilibrium or deviation from Hardy–Weinberg equilibrium was detected among all loci. The eleven markers developed can be valuable tools to conservation genetics of this species across its distributional range.