Improving osteogenesis of calcium phosphate bone cement by incorporating with manganese doped β-tricalcium phosphate
Improving osteogenesis of calcium phosphate bone cement by incorporating with manganese doped β-tricalcium phosphate
复制标题
掺入锰掺杂β-磷酸三钙改善磷酸钙骨水泥的成骨作用
DOI：
10.1016/j.msec.2019.110481
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发表时间：
2020-04-01
影响因子：
7.9
通讯作者：
Ye, Jiandong
中科院分区：
文献类型：
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作者：
Wu, Tingting;Shi, Haishan;Ye, Jiandong
成骨能力的缺乏限制了磷酸钙骨水泥（CPC）的骨修复效果。在本研究中，将二价锰离子（Mn²⁺）掺杂的β - 磷酸三钙（Mn - TCP）掺入CPC以增强其成骨能力。Mn - TCP的掺入促进了CPC的水化反应。Mn²⁺的存在使水化产物更精细。当在CPC中添加10 wt%的Mn - TCP（Mn - CPC - 1）时，CPC的凝固时间缩短，而强度和可注射性没有改变。在Mn - CPC - 1和含有20 wt% Mn - TCP的CPC（Mn - CPC - 2）上的小鼠骨髓间充质干细胞（mBMSCs）表现出更好的黏附和铺展行为。此外，Mn - CPC - 1促进了碱性磷酸酶（ALP）、Ⅰ型胶原蛋白（Col - I）和骨钙素（OC）的基因水平，而Mn - CPC - 2促进了Runx2和OC的基因水平。细胞行为与两点有关：一是改变骨水泥表面性质后蛋白质（如牛血清白蛋白，BSA）吸附能力的增加；二是CPC释放的Mn²⁺在成骨过程中的生物学作用。所有结果表明，掺入10 wt% Mn - TCP的CPC具有良好的成骨性能和合适的理化性质，将是一种应用于骨再生领域的有前景的生物材料。
Lack of osteogenic capacity limits the bone repair effect of calcium phosphate cement (CPC). In present work, bivalent manganese ion (Mn2+) doped beta-tricalcium phosphate (Mn-TCP) was incorporated into CPC to enhance its osteogenic ability. The incorporation of Mn-TCP promoted the hydration reaction of CPC. The presence of Mn2+ made the hydration products finer. When adding 10 wt% Mn-TCP in CPC (Mn-CPC-1), the setting time of CPC was shortened, whereas the strength and injectability were not changed. Mouse Bone marrow mesenchymal stem cells (mBMSCs) on Mn-CPC-1 and CPC with 20 wt% Mn-TCP (Mn-CPC-2) presented better adhesion and spreading behaviors. Besides, Mn-CPC-1 promoted the gene levels of ALP, Col-I and OC while Mn-CPC-2 promoted the gene levels of Runx2 and OC. Cellular behaviors were related to two points: one was the increase of adsorption capacity of proteins (e.g. BSA) after changing the surface properties of bone cements; and the other was the biological role of Mn2+ released from CPC in osteogenesis. All the results indicated that CPC incorporated with 10 wt% Mn-TCP has good osteogenesis and proper physicochemical properties, which will be a prospective biomaterial applying in the area of bone regeneration.