Particle stability and structure on the peritoneal surface in pressurized intra-peritoneal aerosol chemotherapy (PIPAC) analysed by electron microscopy: First evidence of a new physical concept for PIPAC
Particle stability and structure on the peritoneal surface in pressurized intra-peritoneal aerosol chemotherapy (PIPAC) analysed by electron microscopy: First evidence of a new physical concept for PIPAC
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DOI：
10.3892/ol.2019.10162
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发表时间：
2019-06-01
期刊：
影响因子：
2.9
通讯作者：
Mikolajczyk, Agata
中科院分区：
文献类型：
--
作者：
Khosrawipour, Tanja;Schubert, Justyna;Mikolajczyk, Agata
加压腹膜腔内雾化化疗(PIPAC)作为一种治疗腹膜转移瘤的新方法已被引入临床。化学气雾剂微滴与腹膜表面的局部相互作用及其分布模式被认为是相对于传统的液体腹膜内化疗的主要优势。本研究的目的是通过电子显微镜观察这些气溶胶粒子在PIPAC应用过程中的行为。使用建立的体外模型雾化多西环素、脂质体阿霉素和巨噬细胞的溶液。在27℃下，通过微导管(MC)对腹膜样品进行PIPAC，压力为12 mm HgC0(2)。在PIPAC之后，通过电子显微镜测量施加颗粒的表面结构。多西环素与气雾剂颗粒的接触在腹膜表面形成了一层高度约为200 nm的纳米膜，该高度与初始颗粒的大小无关。这些圆柱体的纳米膜块直径不同，取决于击中该点的初始气溶胶粒子。这些圆柱体的直径比最初的气溶胶粒子的原始直径要宽得多。然而，脂质体阿霉素和巨噬细胞等包被颗粒在与腹膜表面接触后保持完好。基于这一数据和其他数据，气雾剂颗粒在腹部表现出气体样行为的概念应该得到修订，从而形成治疗性的腹膜。液体气溶胶粒子与腹膜相撞，形成纳米薄膜。因此，加压的腹膜内气雾剂对腹膜的相互作用更接近于液膜的分布，而不是气体的分布。需要进一步的研究来进一步分析这种纳米膜与腹膜的相互作用。
Pressurized intra-peritoneal aerosol chemotherapy (PIPAC) has been introduced to the clinical setting as a novel approach for the treatment of peritoneal metastasis. The local interaction of chemoaerosol droplets with the peritoneal surface as well as their distribution pattern is considered the main advantage over conventional liquid intraperitoneal chemotherapy. The aim of the present study was to investigate the behavior of these aerosol particles during PIPAC application via electron microscopy. Solutions of doxycycline, liposomal doxorubicin and macrophage cells were aerosolized using an established ex-vivo model. PIPAC was performed on peritoneum samples via microcatheter (MC) at a pressure of 12 mmHg C0(2) at 27 degrees C. Following PIPAC the surface structure of applied particles was measured via electron microscopy. The aerosol particle contact of doxycyclin created a nanofilm of similar to 200 nm height on the peritoneal surface, and this height was revealed to be independent of the size of the initial particle hitting. These nanofilm blocks of cylinders' are of different diameters depending on the initial aerosol particle hitting that spot. Diameters of these cylinders' are far wider than the original diameter of the initial aerosol particle. However, coated particles such as liposomal doxorubicin and macrophages remained intact following contact with the peritoneal surface. Based on this and other data, the concept that aerosol particles exhibit a gas-like behavior in the abdomen creating a therapeutic capnoperitoneum should be revised. Fluid aerosol particles collide with the peritoneum creating a nanofilm. The interaction of pressurized intraperitoneal aerosol on the peritoneum is therefore closer to the distribution of a liquid film than to that of a gas. Further studies are required to further analyze the interaction of this nanofilm on the peritoneum.