Chromosomal radiosensitivity during the G2 cell-cycle period of skin fibroblasts from individuals with familial cancer.
Chromosomal radiosensitivity during the G2 cell-cycle period of skin fibroblasts from individuals with familial cancer.
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家族性癌症患者皮肤成纤维细胞 G2 细胞周期期间的染色体放射敏感性。
DOI：
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发表时间：
1985
影响因子：
11.1
通讯作者：
G. Jones
中科院分区：
文献类型：
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作者：
R. Parshad;K. Sanford;G. Jones
我们以前曾报道，在细胞周期的G2期，人类细胞经过肿瘤转化后，对x射线引起的染色单体损伤的敏感性增加。有证据表明，这是由于G2期DNA修复不足所致。来自人类肿瘤的细胞也显示出增强的G2期染色体放射敏感性。此外，来自易患癌症的高风险遗传病的个体的皮肤成纤维细胞，包括共济失调-毛细血管扩张症、布卢姆综合征、范可尼贫血和着色性干皮病，显示出增强的G2期染色体放射敏感性。目前的研究表明，来自家族性癌症患者的表面正常的皮肤成纤维细胞--即来自有肿瘤病史的家庭--也表现出增强的G2期染色体放射敏感性。因此，这种放射敏感性似乎既与癌症的遗传易感性有关，也与恶性肿瘤状态有关。此外，增强的G2期染色体放射敏感性可能为检测癌症遗传易感性的检测提供基础。
We reported previously that human cells after neoplastic transformation in culture had acquired an increased susceptibility to chromatid damage induced by x-irradiation during the G2 phase of the cell cycle. Evidence suggested that this results from deficient DNA repair during G2 phase. Cells derived from human tumors also showed enhanced G2-phase chromosomal radiosensitivity. Furthermore, skin fibroblasts from individuals with genetic diseases predisposing to a high risk of cancer, including ataxia-telangiectasia, Bloom syndrome, Fanconi anemia, and xeroderma pigmentosum exhibited enhanced G2-phase chromosomal radiosensitivity. The present study shows that apparently normal skin fibroblasts from individuals with familial cancer--i.e., from families with a history of neoplastic disease--also exhibit enhanced G2-phase chromosomal radiosensitivity. This radiosensitivity appears, therefore, to be associated with both a genetic predisposition to cancer and a malignant neoplastic state. Furthermore, enhanced G2-phase chromosomal radiosensitivity may provide the basis for an assay to detect genetic susceptibility to cancer.