Endocytosis of liposomes bound to cell surface proteins measured by flow cytofluorometry.
Endocytosis of liposomes bound to cell surface proteins measured by flow cytofluorometry.
复制标题
通过流式细胞荧光法测量与细胞表面蛋白结合的脂质体的内吞作用。
DOI：
10.1042/bj2140189
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
L. Leserman
中科院分区：
文献类型：
--
作者：
A. Truneh;Z. Mishal;J. Barbet;P. Machy;L. Leserman
一种新的技术，用于定量细胞受体介导的内吞作用已经开发的基础上，通过流式细胞术的配体轴承含有荧光染料羧基荧光素的脂质体的分析。高浓度的羧基荧光素包封在蛋白A-轴承脂质体是自淬灭的。细胞通过针对膜表面决定簇的抗体结合和内化这种脂质体，导致脂质体包封的羧基荧光素释放到细胞质中，引起细胞相关荧光的增加。这种增加可以在流式细胞荧光计上定量。
A new technique for the quantification of cellular receptor-mediated endocytosis has been developed based on the analysis by flow cytometry of ligand-bearing liposomes containing the fluorochrome carboxyfluorescein. Carboxyfluorescein encapsulated at high concentrations in protein A-bearing liposomes is self-quenched. Binding and internalization of such liposomes by cells via antibodies directed towards membrane surface determinants results in the release of the liposome-encapsulated carboxyfluorescein into the cytoplasm causing an increase in cell-associated fluorescence. This increase can be quantified on a flow cytofluorometer.