Intracellular activities of sodium and potassium.
Intracellular activities of sodium and potassium.
复制标题
钠和钾的细胞内活性。
DOI：
10.1152/ajprenal.1978.234.4.f261
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
M. Civan
中科院分区：
文献类型：
--
作者：
M. Civan
细胞内Na+和K+的含量似乎调节了多种细胞和组织功能，包括跨上皮运输。然而，这些总离子含量似乎由Na+和K+的不均匀群体组成。只有不超过几个百分点的细胞内Na+和K+看起来是结合的。因此，细胞内离子含量的异质性必然反映了亚细胞的区隔作用。最近在离子选择性微电极的制备和使用方面的技术进步使得在确定这种细胞内区隔的可能位置方面取得了进展。此外，微电极和微吸管的细胞内记录现在提供了一种技术，可以直接监测细胞质内Na+和K+的化学活动作为跨上皮运输状态的函数。这种方法已经提供了有必要重新检查运输生理学某些经典概念的信息。
The intracellular contents of Na+ and K+ appear to modulate a variety of cellular and tissue functions, including transepithelial transport. However these total ionic contents appear to consist of heterogeneous populations of Na+ and K+. No more than a few percent of intracellular Na+ and K+ appear bound. Thus, the heterogeneity of the intracellular ionic contents must reflect subcellular compartmentalization. Recent technical advances in the preparation and use of ion-selective microelectrodes have permitted progress in defining the possible sites of such intracellular compartmentalization. Furthermore, intracellular recording with microelectrodes and micropipettes now provides a technique with which to directly monitor the chemical activities of Na+ and K+ within the cytoplasm as a function of the state of transepithelial transport. This approach has already provided information necessitating a reexamination of certain classical concepts of transport physiology.