ASSOCIATION OF 2'-5' OLIGORIBONUCLEOTIDES
ASSOCIATION OF 2'-5' OLIGORIBONUCLEOTIDES
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DOI：
10.1093/nar/20.7.1685
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发表时间：
1992-04-11
影响因子：
14.9
通讯作者：
TURNER, DH
中科院分区：
文献类型：
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作者：
KIERZEK, R;HE, LY;TURNER, DH
具有2 '-5'键的寡核糖核苷酸已经在固体载体上合成。UV解链和CD实验表明互补链缔合得到的复合物的解链温度比由具有相同序列的3 '-5'寡核糖核苷酸形成的双链体低30 - 40 ℃。（2 '-5'）CGGCGCCG的UV熔解和亚氨基质子NMR光谱和NOE与反平行双链体的形成一致。结果表明，更大的双链体稳定性是进化中有利于3 '-5'而不是2 '-5'键的一个因素。
Oligoribonucleotides with 2'- 5' linkages have been synthesized on solid support. UV melting and CD experiments indicate complementary strands associate to give complexes with melting temperatures 30 to 40-degrees-C lower than for duplexes formed by 3'- 5' oligoribonucleotides with the same sequence. UV melting and imino proton NMR spectra and NOEs for (2'-5') CGGCGCCG are consistent with formation of an antiparallel duplex. The results suggest greater duplex stability was one factor favoring 3'-5' over 2'-5' linkages in evolution.