GENE TRANSCRIPTS FOR THE NICOTINIC ACETYLCHOLINE-RECEPTOR SUBUNIT, BETA4, ARE DISTRIBUTED IN MULTIPLE AREAS OF THE RAT CENTRAL-NERVOUS-SYSTEM
GENE TRANSCRIPTS FOR THE NICOTINIC ACETYLCHOLINE-RECEPTOR SUBUNIT, BETA4, ARE DISTRIBUTED IN MULTIPLE AREAS OF THE RAT CENTRAL-NERVOUS-SYSTEM
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DOI：
10.1016/0169-328x(92)90244-6
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发表时间：
1992-12-01
期刊：
影响因子：
--
通讯作者：
PATRICK, J
中科院分区：
文献类型：
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作者：
DINELEYMILLER, K;PATRICK, J
先前的原位杂交实验报道，β4（β4）神经元烟碱乙酰胆碱受体（nAChR）转录物仅在内侧缰核（MHB）中发现。非洲爪蟾卵母细胞中 β4 亚基和三种配体结合或 α 亚基中任何一种的共表达会导致功能性 nAChR 的形成。卵母细胞中表达的 nAChR 的药理学与大鼠 CNS 中发现的 nAChR 的药理学之间的比较促使人们进一步研究编码 β4 nAChR 亚基的转录本的定位。使用两个β4特异性cRNA探针，在大鼠脑中进行原位杂交。在海马结构的前下托、副下托、下托和齿状回、等皮质第四层、内侧缰核、脚间核和三叉神经运动神经核中检测到高水平的 Beta4 mRNA。在等皮质（I-III 层）、嗅觉区域、阿蒙角的 CA1 至 CA4 区和海马结构的内嗅皮质、乳头上核、脑桥核和小脑中观察到中等杂交信号。和蓝斑。在脑干的隔膜、基底神经节、感觉部分中没有检测到高于背景的杂交。或脊髓。
Previous in situ hybridization experiments reported that beta4 (beta4) neuronal nicotinic acetylcholine receptor (nAChR) transcripts were found only in the medial habenula (MHB). Co-expression in Xenopus oocytes of the beta4 subunit and any one of three ligand-binding or alpha subunits results in the formation of functional nAChRs. Comparisons between the pharmacology of nAChRs expressed in oocytes and the pharmacology of nAChRs found in the rat CNS prompted a further investigation of the localization of transcripts encoding the beta4 nAChR subunit. Using two beta4-specific cRNA probes, in situ hybridization was performed in rat brain. Beta4 mRNA was detected at high levels in the presubiculum, parasubiculum, subiculum and dentate gyrus of the hippocampal formation, in layer IV of the isocortex, in the medial habenula, in the interpeduncular nucleus, and in the trigeminal motor nerve nucleus. Moderate hybridization signals were seen in the isocortex (layers I-III), in olfactory regions, in fields CA1 through CA4 of Ammon's horn and the entorhinal cortex of the hippocampal formation, in the supramammillary nucleus, in the pontine nucleus, in the cerebellum. and in the locus coeruleus. No hybridization above background was detected in the septum, basal ganglia, sensory portions of the brainstem. or spinal cord.