Production of New Antibacterial 4-Hydroxy-α-Pyrones by a Marine Fungus Aspergillus niger Cultivated in Solid Medium
Production of New Antibacterial 4-Hydroxy-α-Pyrones by a Marine Fungus Aspergillus niger Cultivated in Solid Medium
复制标题
固体培养基中培养的海洋真菌黑曲霉生产新型抗菌 4-羟基-α-吡喃酮
DOI：
10.3390/md17060344
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发表时间：
2019-06-01
期刊：
影响因子：
5.4
通讯作者：
Xu, Shihai
中科院分区：
文献类型：
--
作者：
Ding, Lijian;Ren, Lu;Xu, Shihai
从水稻固体培养基中培养的海洋海绵真菌Aspergillus尼日尔中分离得到4个4-羟基-α-吡喃酮，其中3个为新化合物，命名为nipyrones A-C（1-3），另1个为已知的杀菌素类似物germicidin C（4）。通过光谱数据和电子圆二色性（ECD）计算以及与文献数据的比较，确定了它们的结构和绝对构型。评价化合物1-4对五种病原菌（金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌、耐甲氧西林金黄色葡萄球菌和结核分枝杆菌）的抗菌活性。化合物3显示出较好的抗沙门氏菌活性。aureus和B.枯草杆菌的最低抑菌浓度（MIC）分别为8 μ g/mL和16 μ g/mL，对结核分枝杆菌有较弱的抗结核活性。化合物1和2对4种病原菌表现出中等的抗菌效果，MIC值为32-64 μ g/mL。
Four 4-hydroxy-alpha -pyrones including three new ones named nipyrones A-C (1-3) together with one known analogue germicidin C (4) were discovered from a marine sponge-derived fungus Aspergillus niger cultivated in a solid rice culture. Their structures and absolute configurations were elucidated through a combination of spectroscopic data and electronic circular dichroism (ECD) calculations as well as comparison with literature data. Compounds 1-4 were evaluated for their antibacterial activities against five pathogenic bacteria (Staphylococcus aureus, Escherichia coli, Bacillus subtilis, methicillin-resistant Staphylococcus aureus, and Mycobacterium tuberculosis). Compound 3 showed promising activity against S. aureus and B. subtilis, with minimum inhibitory concentration (MIC) values of 8 mu g/mL and 16 mu g/mL, respectively, and displayed weak antitubercular activities against M. tuberculosis, with MIC value of 64 mu g/mL, while compounds 1 and 2 exhibited moderate antibacterial efficacy against four pathogenic bacteria with MIC values of 32-64 mu g/mL.