Rapid and efficient transposon mutagenesis of Bartonella henselae by transposome technology.
Rapid and efficient transposon mutagenesis of Bartonella henselae by transposome technology.
复制标题
利用转座体技术对汉赛巴尔通体进行快速高效的转座子诱变。
DOI：
10.1016/s0378-1119(03)00636-x
复制
发表时间：
2003
期刊：
影响因子：
3.5
通讯作者：
Kempf,VolkhardAJ
中科院分区：
文献类型：
--
作者：
Riess,Tanja;Anderson,Burt;Fackelmayer,Andrea;Autenrieth,IngoB;Kempf,VolkhardAJ
汉赛巴尔通体是猫抓病和血管增生性疾病杆菌性血管瘤病和杆菌性紫癜的病原体，分子遗传学很难进行。为了阐明潜在的细菌致病机制，对B. henselae是首选方法。我们描述了如何在B中进行转座子诱变。利用转座体技术研究henselae。B。通过该技术揭示的henselae突变体显示随机易位插入染色体。与通过接合转移的转座子诱变相反，转座体技术允许转座子诱变早期传代的巴尔通体属物种。效率提高了大约100倍。结果表明，转座体技术是一种快速、高效、简便的获得B转座子突变体的方法。亨瑟莱
Molecular genetics are difficult to perform in Bartonella henselae, the causative agent of cat scratch disease and the vasculoproliferative disorders bacillary angiomatosis and bacillary peliosis. To elucidate the underlying bacterial pathogenic mechanisms, genetic manipulation of B. henselae is the method of choice. We describe how to perform transposon mutagenesis in B. henselae using transposome technology. B. henselae mutants revealed by this technique showed random transpositional insertion into the chromosome. In contrast to transposon mutagenesis by conjugational transfer, transposome technology allows transposon mutagenesis of early passaged Bartonella spp. with approximately 100-fold higher efficiency. The results show that transposome technique is a rapid, efficient and simple method to generate transposon mutants of B. henselae.