Biotransformation of heptachlor and heptachlor epoxide by white-rot fungus Pleurotus ostreatus
Biotransformation of heptachlor and heptachlor epoxide by white-rot fungus Pleurotus ostreatus
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DOI：
10.1016/j.ibiod.2013.02.013
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发表时间：
2013-08-01
影响因子：
4.8
通讯作者：
Kondo, Ryuichiro
中科院分区：
文献类型：
--
作者：
Purnomo, Adi Setyo;Mori, Toshio;Kondo, Ryuichiro
研究了平菇对七氯和环氧七氯的转化能力。在马铃薯葡萄糖肉汤（PDB）和高氮（HN）培养基中培养14 d，七氯被该真菌完全消除：除二氯和1-羟基二氯外，还检测到环氧化七氯作为主要代谢产物。在对代谢产物进行鉴定的基础上，提出了七氯的转化途径，七氯的代谢途径为环氧化、脱氯和水解。该菌还能降解七氯的一种代谢产物--环氧化七氯。在低氮（LN）、HN和PDB培养基中培养14 d，平菇对环氧化七氯的去除率分别为9%、8%和31%。乙酰化后，在PDB培养基培养物中检测到代谢产物2，3-二羟基七氯（七氯二醇），表明水解反应发生在环氧环上。这表明P.流是七氯和七氯环氧化物转化的潜在来源。(C)2013爱思唯尔有限公司保留所有权利。
The ability of Pleurotus ostreatus to transform heptachlor as well as heptachlor epoxide was investigated. Heptachlor was eliminated completely by this fungus in potato dextrose broth (PDB) and high nitrogen (HN) media during a 14 d incubation period: Heptachlor epoxide was detected as the main metabolite besides chlordene and 1-hydoxychlordene. Based on identification of the metabolites, transformation of heptachlor was proposed which was initially metabolized by epoxidation, dechlorination and hydrolysis. This fungus was also capable of degrading heptachlor epoxide which is a recalcitrant metabolite of heptachlor. Approximately 9%, 8% and 31% of heptachlor epoxide was eliminated by P. ostreatus in low nitrogen (LN), HN, and PDB media during 14 days of incubation, respectively. After acetylation, the metabolite, 2,3-dihydroxyheptachlor (Heptachlor diol), was detected in the PDB medium cultures, suggesting the hydrolysis reaction occurred in the epoxide ring. This indicated that P. streams is a potential source for transformation of heptachlor as well as heptachlor epoxide. (C) 2013 Elsevier Ltd. All rights reserved.