Ascorbate peroxidase gene family in tomato: its identification and characterization
Ascorbate peroxidase gene family in tomato: its identification and characterization
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DOI：
10.1007/s00438-007-0305-2
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发表时间：
2008-02-01
影响因子：
3.1
通讯作者：
Volokita, Micha
中科院分区：
文献类型：
--
作者：
Najami, Naim;Janda, Tibor;Volokita, Micha
抗氧化反应，其中抗坏血酸过氧化物酶（APX）是一个关键的酶，是一个不可分割的一部分，植物耐受性的反应，环境压力。作为研究Apx基因家族成员的生理作用和调控的第一步，胁迫敏感栽培番茄Solanum lycopersicum cv. M82（Slm）和野生耐盐种S.利用番茄EST数据库鉴定并鉴定了Pennellii acc.Atico（Spa）的11个种属。16个虚拟Apx转录本和4个单例EST的冗余列表显示对应于7个真正的Apx基因。完整的番茄APX基因家族由编码三个胞质APX、两个过氧化物酶体APX和两个叶绿体APX的基因组成。这些基因在不同的SLM器官中获得不同的调控模式。更详细的研究Apx1和Apx2基因，这是最近的基因复制事件的产物，表明他们已经达到了控制和压力条件下的Slm和Spa之间的差异调节。同时也表明，由于谱系特异性的基因重复和丢失事件，Apx基因家族成员之间存在着复杂的系统发育关系。
The antioxidative response, where ascorbate peroxidase (APX) is a key enzyme, is an integral part of the plant tolerance response to environmental stresses. As a first step towards the study of the physiological role and the regulation of the members of the Apx gene family, the orthologs of the stress-sensitive cultivated tomato Solanum lycopersicum cv. M82 (Slm) and of the wild salt-tolerant species S. pennellii acc. Atico (Spa) were identified by utilizing the tomato EST database, and characterized. A redundant list of 16 virtual Apx transcripts and four singleton ESTs was shown to correspond to seven genuine Apx genes. The complete tomato Apx gene family is comprised of genes encoding three cytosolic, two peroxisomal, and two chloroplastic APXs. These genes attained differential regulatory patterns in various Slm organs. More detailed study of Apx1 and Apx2 genes, that are the products of a recent gene duplication event, shows that they have already attained differential regulation within and between Slm and Spa under control and stress conditions. It is also suggested that due to lineage-specific gene duplication and lose events, intricate phylogenetic relationships exist among the members of the Apx gene families.