Innate immune markers in mothers and fathers of children newly diagnosed with cancer
Innate immune markers in mothers and fathers of children newly diagnosed with cancer
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DOI：
10.1159/000148192
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发表时间：
2008-01-01
影响因子：
2.4
通讯作者：
Douglas, Steven D.
中科院分区：
文献类型：
--
作者：
Stehl, Meredith J. Lutz;Kazak, Anne E.;Douglas, Steven D.
目的：对父母来说，诊断出孩子患有威胁生命的疾病是最紧张的事情之一，这与焦虑和痛苦的增加有关。尽管在动物和人类模型中，压力与免疫功能之间存在关联，但在发生与儿童有关的潜在创伤事件（如癌症）时，儿童照料者的免疫功能尚不清楚。方法：19对父母（11名母亲，8名父亲），代表6对照顾者，在得知孩子患有癌症后的2周内，通过流式细胞术和功能测定（% NK全血，绝对NK全血，LU20（溶解单位）外周血单个核细胞，LU20 NK细胞）提供了自然杀伤（NK）细胞计数的血液，并完成了自我报告（急性应激）。NK细胞测定也用健康成人样本（免疫参照组）完成。结果：护理者与免疫参照组之间NK细胞活性水平相似。免疫水平与心理结果无关。LU20外周血单个核细胞和LU20 NK细胞在同一家庭中与母亲和父亲的相关性均为r = 0.83。结论：虽然基于小样本，但这些初步结果表明，了解患有危及生命疾病的儿童父母的压力反应可能是重要的，并提供了关于压力对照顾者夫妇免疫功能共同影响的新数据。版权所有2008 S. Karger AG，巴塞尔
Objective: The diagnosis of a life-threatening illness in a child is one of the most stressful events imaginable for parents and is associated with increased anxiety and distress. Despite associations between stress and immune function in animal and human models, the immune function in caregivers of children at the time of a child-related potentially traumatic event, like cancer, is not known. Methods: Nineteen parents (11 mothers, 8 fathers), representing six caregiver pairs, provided blood for natural killer (NK) cell count by flow cytometry and function assays [% NK whole blood, absolute NK whole blood, LU20 (lytic unit) peripheral blood mononuclear cells, LU20 NK cells] and completed self-report measures (acute stress) within 2 weeks of learning their child had cancer. The NK cell assay was also completed with a sample of healthy adults, the immune reference group. Results: There were similar levels of NK cell activity between caregivers and the immune reference group. Immune level and psychological outcomes were not associated. LU20 peripheral blood mononuclear cells and LU20 NK cells were each correlated at r = 0.83 between mothers and fathers in the same family. Conclusions: Although based on a small sample, these preliminary results suggest that knowledge about stress responses in parents of children with life-threatening illness may be important and provide novel data regarding the shared impact of stress on immune function within caregiver dyads. Copyright (C) 2008 S. Karger AG, Basel.