MicroRNA and tasiRNA diversity in mature pollen of Arabidopsis thaliana.
MicroRNA and tasiRNA diversity in mature pollen of Arabidopsis thaliana.
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DOI：
10.1186/1471-2164-10-643
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发表时间：
2009-12-30
期刊：
影响因子：
4.4
通讯作者：
Dickinson H
中科院分区：
文献类型：
--
作者：
Grant-Downton R;Le Trionnaire G;Schmid R;Rodriguez-Enriquez J;Hafidh S;Mehdi S;Twell D;Dickinson H
新一代测序技术已经允许对开花植物的小RNA群体进行深入研究。然而，很少有人知道小RNA在其生殖细胞，特别是在减数分裂后的配子体细胞代。花粉-雄配子体-是一种特殊的单倍体结构，在受精时产生精子并将其传递给雌配子。雄配子体的发育和分化是否取决于指导基因表达变化的microRNA和反式作用siRNA的作用在很大程度上是未知的。在这里，我们已经使用454测序调查各种小RNA群体存在于成熟的花粉拟南芥。在这项研究中，我们检测了成熟花粉中存在的33个不同的microRNA家族，并通过Q-RT-PCR验证了17个选择的miRNA的表达水平。与叶片相比，大多数选择的miRNA显示花粉富集表达。此外，我们首次报告了花粉中存在反式作用siRNA。除了描述这些类别中已知小RNA的新表达模式外，我们还鉴定了7种推定的新型microRNA。其中之一，ath-MIR 2939，针对花粉特异性的F盒转录本，我们证明了其目标mRNA在成熟花粉中的切割。尽管雄性配子体明显简单，仅包含两种不同的细胞类型，但花粉不仅利用在体细胞组织中表达的许多miRNA和反式作用siRNA，而且还表达新的miRNA。
New generation sequencing technology has allowed investigation of the small RNA populations of flowering plants at great depth. However, little is known about small RNAs in their reproductive cells, especially in post-meiotic cells of the gametophyte generation. Pollen - the male gametophyte - is the specialised haploid structure that generates and delivers the sperm cells to the female gametes at fertilisation. Whether development and differentiation of the male gametophyte depends on the action of microRNAs and trans-acting siRNAs guiding changes in gene expression is largely unknown. Here we have used 454 sequencing to survey the various small RNA populations present in mature pollen of Arabidopsis thaliana. In this study we detected the presence of 33 different microRNA families in mature pollen and validated the expression levels of 17 selected miRNAs by Q-RT-PCR. The majority of the selected miRNAs showed pollen-enriched expression compared with leaves. Furthermore, we report for the first time the presence of trans-acting siRNAs in pollen. In addition to describing new patterns of expression for known small RNAs in each of these classes, we identified 7 putative novel microRNAs. One of these, ath-MIR2939, targets a pollen-specific F-box transcript and we demonstrate cleavage of its target mRNA in mature pollen. Despite the apparent simplicity of the male gametophyte, comprising just two different cell types, pollen not only utilises many miRNAs and trans-acting siRNAs expressed in the somatic tissues but also expresses novel miRNAs.
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DOI：
10.1073/pnas.162339999
发表时间：
2002-08-20
影响因子：
11.1
作者：
Gagne, JM;Downes, BP;Vierstra, RD
通讯作者：
Vierstra, RD
影响因子：
11.6
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通讯作者：
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影响因子：
11.6
作者：
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影响因子：
7.2
作者：
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通讯作者：
Russell, Scott D.
影响因子：
9.2
作者：
Fang, Yuda;Spector, David L.
通讯作者：
Spector, David L.