FUNCTION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 VPU PROTEIN IN VARIOUS CELL-TYPES
FUNCTION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 VPU PROTEIN IN VARIOUS CELL-TYPES
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DOI：
10.1099/0022-1317-76-11-2717
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发表时间：
1995-11-01
影响因子：
3.8
通讯作者：
ADACHI, A
中科院分区：
文献类型：
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作者：
SAKAI, H;TOKUNAGA, K;ADACHI, A
我们评估了人类免疫缺陷病毒1型VPU基因在不同细胞系中的功能。我们建立了由氯霉素乙酰转移酶和逆转录酶检测组成的高灵敏体系，并用它来监测VPU基因突变的影响。在一些细胞系中，VPU蛋白在病毒复制的早期阶段不是必需的，但对于有效地产生病毒粒子是重要的。在这些细胞中，无论是否存在完整的env基因产物，VPU蛋白都能有效发挥作用。同样，CD4基因表达对VPU功能无影响。在测试的其他细胞系中，VPU蛋白对病毒粒子的释放并不重要，VPU突变克隆在转染后产生了正常水平的子代病毒粒子。
We evaluated the function of human immunodeficiency virus type 1 vpu gene in various cell lines. We established a highly sensitive system consisting of chloramphenicol acetyltransferase and reverse transcriptase assays and used it to monitor the effects of mutation of the vpu gene. In some cell lines, Vpu protein was not required at the early phase of viral replication but was important for efficient virion production. In these cells, the Vpu protein functioned effectively irrespective of the presence of intact env gene products. Likewise, CD4 gene expression had no effects on Vpu function. In the other cell lines tested, Vpu protein was not important for virion release, and the vpu mutant clone generated a normal level of progeny virions upon transfection.