In vivo time-lapse imaging reveals extensive neural crest and endothelial cell interactions during neural crest migration and formation of the dorsal root and sympathetic ganglia
In vivo time-lapse imaging reveals extensive neural crest and endothelial cell interactions during neural crest migration and formation of the dorsal root and sympathetic ganglia
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DOI：
10.1016/j.ydbio.2016.02.028
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发表时间：
2016-05-01
影响因子：
2.7
通讯作者：
Lefcort, Frances
中科院分区：
文献类型：
--
作者：
George, Lynn;Dunkel, Haley;Lefcort, Frances
在羊膜胚胎发生过程中，神经系统和血管系统相互作用，通过形成“神经血管”连接，显著影响每个网络各自的形态发生。随着人们越来越多地认识到这两个系统在发育过程、干细胞生态位以及阿尔茨海默病和肌萎缩性侧索硬化症等神经退行性疾病中广泛相互作用，神经血管相互作用在中枢神经系统中的重要性最近受到关注。然而，对于周围神经系统，还没有对这两个发育系统之间潜在关系的实时调查。为了解决这一缺陷，我们在转基因鹌鹑模型中使用了多光谱4D延时成像，其中内皮细胞（ECs）表达黄色荧光标记，而神经嵴细胞（NCCs）表达电穿孔红色荧光标记。我们在周围神经系统形成过程中实时监测EC和NCC的迁移。我们的延时记录表明，ncc和ec在物理上并置，并在其轨迹的多个位置动态相互作用。这些相互作用是典型的，发生在沿NCC迁移途径的精确解剖位置。ncc沿着发育中的体间血管的后表面移动，但不能穿过这些连续的运动ECs流。当ncc在发育中的脉管系统中遇到间隙时，它们会改变形态和迁移轨迹。在新生的背根神经节内，靠近内皮细胞引起丝状回收缩，从而限制了NCC向前运动的持久性。总的来说，我们的延时记录支持初级血管网络实质上影响ncc的分布和迁移行为以及背根和交感神经节的模式形成的结论。Elsevier Inc.出版。
During amniote embryogenesis the nervous and vascular systems interact in a process that significantly affects the respective morphogenesis of each network by forming a ''neurovascular" link. The importance of neurovascular cross-talk in the central nervous system has recently come into focus with the growing awareness that these two systems interact extensively both during development, in the stem-cell niche, and in neurodegenerative conditions such as Alzheimer's Disease and Amyotrophic Lateral Sclerosis. With respect to the peripheral nervous system, however, there have been no live, real-time investigations of the potential relationship between these two developing systems. To address this deficit, we used multi spectral 4D time-lapse imaging in a transgenic quail model in which endothelial cells (ECs) express a yellow fluorescent marker, while neural crest cells (NCCs) express an electroporated red fluorescent marker. We monitored EC and NCC migration in real-time during formation of the peripheral nervous system. Our time-lapse recordings indicate that NCCs and ECs are physically juxtaposed and dynamically interact at multiple locations along their trajectories. These interactions are stereotypical and occur at precise anatomical locations along the NCC migratory pathway. NCCs migrate alongside the posterior surface of developing intersomitic vessels, but fail to cross these continuous streams of motile ECs. NCCs change their morphology and migration trajectory when they encounter gaps in the developing vasculature. Within the nascent dorsal root ganglion, proximity to ECs causes filopodial retraction which curtails forward persistence of NCC motility. Overall, our time-lapse recordings support the conclusion that primary vascular networks substantially influence the distribution and migratory behavior of NCCs and the patterned formation of dorsal root and sympathetic ganglia. Published by Elsevier Inc.