Scaffold-free inkjet printing of three-dimensional zigzag cellular tubes
Scaffold-free inkjet printing of three-dimensional zigzag cellular tubes
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DOI：
10.1002/bit.24591
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发表时间：
2012-12-01
影响因子：
3.8
通讯作者：
Markwald, Roger R.
中科院分区：
文献类型：
--
作者：
Xu, Changxue;Chai, Wenxuan;Markwald, Roger R.
打印三维(3D)细胞管的能力不仅是迈向成功器官打印的合乎逻辑的第一步，而且也是设想的器官打印技术可行性的关键指标。已经提出了一种平台辅助的3D喷墨生物打印系统来制造3D复杂结构，如之字形管。利用所提出的生物打印系统，成功地制造了具有悬垂结构的成纤维细胞(3T3细胞)基管。即使在确定的良好液滴形成的印刷条件下，印刷细胞管的印刷后3T3细胞存活率仍可达到82%以上(或在考虑控制效果的情况下为93%)，这表明所提出的生物印刷系统具有良好的应用前景。特别是证明了采用喷墨技术可以实现无脚手架的管状悬挑结构，其最大可达高度取决于悬挑结构的倾角。作为一项概念验证研究，由此产生的制造知识有助于打印具有复杂几何结构的组织工程血管。生物技术。比昂斯。2012年；109：31523160。(C)2012年威利期刊公司。
The capability to print three-dimensional (3D) cellular tubes is not only a logical first step towards successful organ printing but also a critical indicator of the feasibility of the envisioned organ printing technology. A platform-assisted 3D inkjet bioprinting system has been proposed to fabricate 3D complex constructs such as zigzag tubes. Fibroblast (3T3 cell)-based tubes with an overhang structure have been successfully fabricated using the proposed bioprinting system. The post-printing 3T3 cell viability of printed cellular tubes has been found above 82% (or 93% with the control effect considered) even after a 72-h incubation period using the identified printing conditions for good droplet formation, indicating the promising application of the proposed bioprinting system. Particularly, it is proved that the tubular overhang structure can be scaffold-free fabricated using inkjetting, and the maximum achievable height depends on the inclination angle of the overhang structure. As a proof-of-concept study, the resulting fabrication knowledge helps print tissue-engineered blood vessels with complex geometry. Biotechnol. Bioeng. 2012; 109: 31523160. (C) 2012 Wiley Periodicals, Inc.