Expression of Ten-m/Odz3 in the fibrous layer of mandibular condylar cartilage during postnatal growth in mice
Expression of Ten-m/Odz3 in the fibrous layer of mandibular condylar cartilage during postnatal growth in mice
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DOI：
10.1111/j.1469-7580.2010.01267.x
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发表时间：
2010-09-01
影响因子：
2.4
通讯作者：
Takano-Yamamoto, Teruko
中科院分区：
文献类型：
--
作者：
Murakami, Takashi;Fukunaga, Tomohiro;Takano-Yamamoto, Teruko
已经推测，下颌骨的发育是通过覆盖在下颌骨上的成纤维细胞样细胞分化成软骨细胞来实现的，然而，这一过程背后的发育机制还没有被揭示。我们使用激光捕获显微切割和cDNA微阵列分析来阐明在这些成纤维细胞样细胞中高表达的基因。其中，Ten-m/Odz3基因在成纤维细胞样细胞中的转录水平显著高于其他软骨组织。本研究首次用原位杂交的方法研究了发育早期的小鼠下颌骨和股骨软骨中Ten-m/Odz3基因的表达与I型、II型和X型胶原基因表达的关系。Ten-m/Odz3在纤维层、增殖期和成熟期软骨细胞层表达，分别表达I型和II型胶原，而在肥大软骨细胞层未见表达。此外，我们利用小鼠成软骨细胞系ATDC5细胞体外表达Ten-m/Odz3。Ten-m/Odz3在间充质细胞向软骨细胞分化的早期表达。这些发现表明，Ten-m/Odz3参与了软骨细胞的分化，并在下颌骨软骨发育的早期阶段起到了调节作用。
It has been speculated that the mandibular condyle develops via the differentiation of the fibroblast-like cells covering the condyle into chondrocytes; however, the developmental mechanisms behind this process have not been revealed. We used laser-capture microdissection and cDNA microarray analysis to elucidate the genes that are highly expressed in these fibroblast-like cells. Among these genes, the transcription of Ten-m/Odz3 was significantly increased in the fibroblast-like cells compared with other cartilage tissues. For the first time, we describe the temporal and spatial expression of Ten-m/Odz3 mRNA in relation to the expression of type I, II, and X collagen mRNA, as determined by in-situ hybridization in mouse mandibular condylar cartilage and mouse femoral cartilage during the early stages of development. Ten-m/Odz3 was expressed in the fibrous layer and the proliferating and mature chondrocyte layers, which expressed type I and II collagen, respectively, but was not detected in the hypertrophic chondrocyte layer. Furthermore, we evaluated the in-vitro expression of Ten-m/Odz3 using ATDC5 cells, a mouse chondrogenic cell line. Ten-m/Odz3 was expressed during the early stage of the differentiation of mesenchymal cells into chondrocytes. These findings suggest that Ten-m/Odz3 is involved in the differentiation of chondrocytes and that it acts as a regulatory factor in the early stages of the development of mandibular condylar cartilage.