Inhibitory Effect of Polysaccharides from Scutellaria barbata D. Don on Invasion and Metastasis of 95-D Cells Lines via Regulation of C-MET and E-CAD Expressions
Inhibitory Effect of Polysaccharides from Scutellaria barbata D. Don on Invasion and Metastasis of 95-D Cells Lines via Regulation of C-MET and E-CAD Expressions
复制标题
半枝莲多糖通过调节C-MET和E-CAD表达抑制95-D细胞系的侵袭和转移
DOI：
10.4314/tjpr.v12i4.11
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发表时间：
2013-08-01
影响因子：
0.6
通讯作者：
Wu, Jin-Jin
中科院分区：
文献类型：
--
作者：
Yang, Xiao-Kun;Yang, Ya-Dong;Wu, Jin-Jin
目的：探讨半枝莲多糖（PSB）对肺癌侵袭和转移的抑制作用，并探讨其可能机制。方法：PSB经水醇沉提取，DEAE-52柱层析纯化。该研究使用了一种高度侵袭性和转移性肺癌细胞95-D细胞系。进行体外细胞粘附和侵袭实验，以评价PSB（50-200μg/ml）对95-D细胞的抗侵袭和抗转移作用。采用免疫细胞化学染色和Western blot技术研究PSB对C-MET和ECAD表达的调节作用。结果：结果表明，PSB 以浓度依赖性方式显着抑制 95-D 的细胞侵袭和迁移（p < 0.05）。 PSB 也抑制 95-D 细胞与纤连蛋白的粘附（p < 0.05）。 PSB处理的95-D细胞中C-MET和E-CAD的表达分别显着下调和上调（p <0.05）。结论：PSB治疗可显着抑制体外95-D细胞的侵袭和转移，其机制可能是通过调节C-MET和E-CAD来实现的。关键词：多糖，半枝莲，95-D细胞系，侵袭，转移
Purpose: To investigate the inhibitory effect of polysaccharides from Scutellaria barbata (PSB) on invasion and metastasis of lung cancer, and study the possible mechanism. Methods: PSB was extracted with water and by alcohol precipitation, and purified by DEAE-52 column chromatography. A highly invasive and metastatic lung carcinoma cell, 95-D cell line, was used for the study. Cell adhesion and invasion assays in vitro were performed to evaluate the anti-invasive and antimetastatic effects of PSB (50 - 200 μg/ml) on 95-D cell. Immunocytochemical staining and Western blot techniques were employed to study the regulatory effects of PSB on the expression of C-MET and ECAD. Results : The results indicate that PSB significantly inhibited cell invasion and migration of 95-D in a concentration-dependent manner (p < 0.05). The adhesion of 95-D cells to fibronectin was also inhibited by PSB (p < 0.05). The expression of C-MET and E-CAD in 95-D cells treated with PSB were significantly down-regulated and up-regulated, respectivelt (p < 0.05). Conclusion: Treatment with PSB can significantly inhibit the invasion and metastasis of 95-D cells in vitro, probably through the regulation of C-MET and E-CAD. Keywords : Polysaccharide, Scutellaria barbata , 95-D cell lines, Invasion, Metastasis