A revised Racine's scale for PTZ-induced seizures in rats
A revised Racine's scale for PTZ-induced seizures in rats
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DOI：
10.1016/j.physbeh.2009.09.005
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发表时间：
2009-12-07
影响因子：
2.9
通讯作者：
van Luijtelaar, Gilles
中科院分区：
文献类型：
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作者：
Luttjohann, Annika;Fabene, Paolo F.;van Luijtelaar, Gilles
行为评分通常用于评估不同癫痫发作模型中的癫痫发作强度。拉辛量表最初是为杏仁核点燃模型开发的，也经常被用作其他实验性癫痫发作或癫痫模型的强度测量。本研究的目的是评估拉辛量表作为戊四唑（PTZ）模型的适当癫痫发作强度测量的有效性。雄性成年Wistar大鼠接受全身注射PTZ，起始剂量为20 mg/kg，每15分钟增加10 mg/kg，直至发生持续5分钟的惊厥发作。同时进行EEG和视频记录。PTZ诱导的癫痫发作强度逐渐增加。11个行为类别进行了识别和统计分析。六种不同的癫痫发作强度类别被发现彼此不同的基础上的差异，在高或低剂量的PTZ和EEG模式的发生模式。这些类别与拉辛的量表有很大的不同。我们认为，拉辛的规模是不够的评估癫痫发作强度的PTZ诱导的癫痫发作，并提出了6个行为癫痫发作类别的替代规模是一个更充分的描述PTZ诱导的癫痫发作。(C)2009 Elsevier Inc. All rights reserved.
Behavioral scoring is commonly used to access seizure intensity in different seizure models. Racine's scale, originally developed for the amygdala-kindling model, is also frequently used as an intensity measurement in other experimental seizure or epilepsy models. The aim of the present study is to assess the validity of Racine's scale as an adequate seizure intensity measurement for the Pentylenetetrazole (PTZ) model. Male adult Wistar rats received systemic injections of PTZ starting with an initial dose of 20 mg/kg added up by 10 mg/kg every 15 min until the occurrence of a 5 minute lasting convulsive seizure. Simultaneous EEG and video recordings were made. The PTZ-induced seizures gradually increased in intensity. Eleven behavioral categories were identified and statistically analyzed. Six different seizure intensity categories were found to differ from each other based on differences in onset latencies, the pattern of occurrence during high or low doses of PTZ and the EEG pattern. These categories were quite different from those of Racine's scale. We suggest that Racine's scale is not adequate for the assessment of the seizure intensity of PTZ-induced seizures and that an alternative scale with the six proposed behavioral seizure categories is a more adequate description of PTZ-induced seizures. (C) 2009 Elsevier Inc. All rights reserved.