Serodiagnosis of Japanese encephalitis among Nepalese patients by the particle agglutination assay
Serodiagnosis of Japanese encephalitis among Nepalese patients by the particle agglutination assay
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DOI：
10.1017/s0950268803008835
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发表时间：
2003-10-01
影响因子：
4.2
通讯作者：
Kurane, I
中科院分区：
文献类型：
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作者：
Pandey, B;Yamamoto, A;Kurane, I
日本脑炎（JE）是尼泊尔西南部地区的一个严重的健康问题。该地区尚未提供用于常规诊断的血清学诊断试剂盒。本研究旨在检验日本脑炎病毒（JEV）IgM 颗粒凝集（PA）测定是否适用于在尼泊尔采集的样本，并评估日本脑炎临床诊断的准确性。 2000年，在尼泊尔西南部乙脑流行地区，采集了临床诊断为乙脑或其他传染病的患者的193份血液样本，对这193份血清样本进行了PA测定，并将结果与IgM捕获ELISA结果进行了比较。 PA 检测显示 86 个样本呈 IgM 阳性，IgM 捕获 ELISA 检测显示 86 个样本中的 71 个也呈阳性（敏感性，99%；特异性，88%；阳性预测值，0.82；阴性预测值，0.99）。这些结果表明，PA 测定是一种简单、可靠且有用的诊断测试，可支持包括尼泊尔在内的亚洲乡村医院的临床诊断。
Japanese encephalitis (JE) is a serious health problem in the southwestern region of Nepal. Serological diagnostic kits for routine diagnostic use in this region have not been available. This study was performed to examine if the particle agglutination (PA) assay for Japanese encephalitis virus (JEV) IgM could be applicable to the samples collected in Nepal and also to evaluate the accuracy of clinical diagnosis of JE. One hundred and ninety-three blood samples were collected from the patients clinically diagnosed with JE or other infectious diseases in the JE-endemic, southwestern region of Nepal, in 2000. The PA assay was performed on these 193 serum samples and the results were compared with those by IgM-capture ELISA. Eighty-six samples were IgM-positive by the PA assay, and 71 of 86 were also positive by IgM-capture ELISA (sensitivity, 99%; specificity, 88%; positive predictive value, 0.82; negative predictive value, 0.99). These results suggest that the PA assay is a simple, reliable and useful diagnostic test to support clinical diagnosis in rural hospitals of Asia including Nepal.