Inhibition of superoxide and nitric oxide release and protection from reoxygenation injury by ebselen in rat kupffer cells
Inhibition of superoxide and nitric oxide release and protection from reoxygenation injury by ebselen in rat kupffer cells
复制标题
依布硒啉抑制大鼠库普弗细胞中超氧化物和一氧化氮的释放并防止再氧合损伤
作者：
Ji;P. Komarov;H. Sies;H. de Groot
Luminol chemiluminescence in phorbolesteractivated cultured rat liver Kupffer cells was strongly inhibited by the selenoorganic compound ebselen (IC50 = 2 μmol/L). Ebselen (2‐phenyl‐1,2‐benzisoselenazol‐3[2H]one) also diminished reduction of ferricytochrome c (IC50 = 10μmol/L), indicating a suppression of superoxide anion formation. Likewise, in lipopolysaccharide‐pretreated Kupffer cells, ebselen proved to be a potent inhibitor of the conversion of oxyhemoglobin to methemoglobin (IC50 = 3 μmol/L) as a measure of nitric oxide formation. The sulfurcontaining analog (2‐phenyl‐1,2‐benzisothiazol‐3[2H]one) and the ebselen derivative, methylselenobenzanilide, were inactive. These results indicate that ebselen is a potent inhibitor of NADPH oxidase in Kupffer cells, as has been reported for other macrophages and granulocytes. In addition, they suggest a novel characteristic of ebselen, namely very effective inhibition of nitric oxide synthase of macrophages. In line with its inhibitory effects on the release of reactive oxygen species by macrophages, complemented by its antioxidant properties, ebselen was potent in the prevention of reoxygenation injury of Kupffer cells (IC50 ∼ 5 μmol/L). (HEPATOLOGY 1992;15:1112–1116).
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影响因子：
5.8
作者：
COTGREAVE, IA;DUDDY, SK;MOLDEUS, P
通讯作者：
MOLDEUS, P
共培养中库普弗细胞对肝细胞的细胞毒性需要 L-精氨酸。
DOI：
10.1001/archsurg.1989.01410120062013
发表时间：
1989
期刊：
Archives of surgery (Chicago, Ill. : 1960)
影响因子：
--
作者：
Billiar,TR;Curran,RD;West,MA;Hofmann,K;Simmons,RL
通讯作者：
Simmons,RL
将蛋白质引入活细胞的电注射方法的评估。
DOI：
10.1152/ajpcell.1991.260.2.c355
发表时间：
1991
期刊：
The American journal of physiology
影响因子：
--
作者：
Wilson,AK;Horwitz,J;DeLanerolle,P
通讯作者：
DeLanerolle,P
影响因子：
--
作者：
JAESCHKE, H;FARHOOD, A
通讯作者：
FARHOOD, A