SEQUENCE HETEROGENEITY AND ANOMALOUS ELECTROPHORETIC MOBILITY OF KINETOPLAST MINICIRCLE DNA FROM LEISHMANIA-TARENTOLAE
SEQUENCE HETEROGENEITY AND ANOMALOUS ELECTROPHORETIC MOBILITY OF KINETOPLAST MINICIRCLE DNA FROM LEISHMANIA-TARENTOLAE
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DOI：
10.1016/0378-1119(84)90071-4
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发表时间：
1984-01-01
期刊：
影响因子：
3.5
通讯作者：
SIMPSON, L
中科院分区：
文献类型：
--
作者：
KIDANE, GZ;HUGHES, D;SIMPSON, L
将来自 L. tarentolae 动质体 DNA 的几个单位长度的小环克隆到 pBR322 和 M13 噬菌体载体中。获得了3个不同的部分同源小环的完整核苷酸序列。这些分子包含约 .apprx 的区域。 80% 序列同源性延伸至 160-270 bp [碱基对]，并且每个小环都有一个独特的区域。发现 14 聚体在迄今为止报道的所有动质体小环序列中都是保守的。 L. tarentolae中各种小环序列类别的频率分布是通过定量凝胶电泳和通过检查在几个位点随机克隆到M13中的小环的T梯模式获得的。这些方法可以分配.apprx。将总小环 DNA 的 50% 分成至少 5 个序列类别。在几个克隆和非克隆的小环片段中观察到序列依赖性聚丙烯酰胺凝胶迁移异常。该异常取决于小环保守区域的一部分的存在。
Several unit-length minicircles from the kinetoplast DNA of L. tarentolae were cloned into pBR322 and into M13 phage vectors. The complete nucleotide sequences of 3 different partially homologous minicircles were obtained. The molecules contained a region of .apprx. 80% sequence homology extending for 160-270 bp [base pair] and a region unique to each minicircle. A 14-mer was found to be conserved in all kinetoplast minicircle sequences reported to date. The frequency distributions of various minicircle sequence classes in L. tarentolae were obtained by quantitative gel electrophoresis and by examination of the T ladder patterns of minicircles randomly cloned into M13 at several sites. These methods could assign .apprx. 50% of the total minicircle DNA into a minimum of 5 sequence classes. A sequence-dependent polyacrylamide gel migration abnormality was observed with several minicircle fragments both cloned and uncloned. The abnormality was dependent on the presence of a portion of the conserved region of the minicircle.