A combined strategy for glycan profiling: a model study with pyridylaminated oligosaccharides.
A combined strategy for glycan profiling: a model study with pyridylaminated oligosaccharides.
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聚糖分析的组合策略：吡啶胺化寡糖的模型研究。
DOI：
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
J. Hirabayashi
中科院分区：
文献类型：
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作者：
Natsuko Kamekawa;Ko Hayama;Sachiko Nakamura;A. Kuno;J. Hirabayashi
结构糖组学在糖科学和糖技术中起着基础性的作用。在本文中，描述了一种新的策略的聚糖的结构表征，其中MS 2分析涉及的LIFT-TOF/TOF程序相结合的前端亲和层析（FAC）。作为模型化合物，选择了20个中性吡啶胺化（PA）寡糖，其中包括四组不同的序列，连接，位置，或分支功能的结构异构体。通过描绘MS 2上的重要诊断离子，成功区分了大多数分析的寡糖，而两对连接异构体，即，LNT/LNnT和LNH/LNnH则无。对于随后的FAC分析，筛选出14种对LNT（1型）或LNnT（2型）具有显著亲和力的凝集素，并将来自海绵Geodia cydonium（GC 1）的半乳糖凝集素和来自蓖麻（Ricinus communis）的植物种子凝集素（RCA-I）分别用于确定1型和2型链。使用这些特异性探针，两种异构体对都被明确区分。此外，一对三触角，天冬酰胺连接的寡糖异构体也可以成功区分。因此，MS 2和FAC的组合是复杂聚糖结构表征的实用替代方案。
Structural glycomics plays a fundamental role in glycoscience and glycotechnology. In this paper, a novel strategy for the structural characterization of glycans is described, in which MS2 analysis involving a LIFT-TOF/TOF procedure is combined with frontal affinity chromatography (FAC). As model compounds, 20 neutral pyridylaminated (PA) oligosaccharides were chosen, which included four groups of structural isomers differing in sequence, linkage, position, or branching features. By depicting significant diagnostic ions on MS2, most of the analyzed oligosaccharides were successfully differentiated, while two pairs of linkage isomers, i.e., LNT/LNnT, and LNH/LNnH were not. For subsequent analysis by FAC, 14 lectins showing significant affinity to either LNT (type 1) or LNnT (type 2) were screened, and a galectin from the marine sponge Geodia cydonium (GC1) and a plant seed lectin from Ricinus communis (RCA-I) were used for determination of type 1 and 2 chains, respectively. With these specific probes, both of the isomeric pairs were unambiguously differentiated. Furthermore, a pair of triantennary, asparagine-linked oligosaccharide isomers could also be successfully differentiated. Thus, the combination of MS2 and FAC is a practical alternative for the structural characterization of complex glycans.
DOI：
10.1016/s0021-9258(18)33746-3
发表时间：
1982-10
期刊：
The Journal of biological chemistry
影响因子：
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作者：
R. Cummings;S. Kornfeld
通讯作者：
R. Cummings;S. Kornfeld