Determination of essential and toxic elements in Cordyceps kyushuensis Kawam by inductively coupled plasma mass spectrometry.
Determination of essential and toxic elements in Cordyceps kyushuensis Kawam by inductively coupled plasma mass spectrometry.
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DOI：
10.1016/j.jpba.2012.08.007
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发表时间：
2013-01
影响因子：
3.4
通讯作者：
Guoying Zhang;Yanxi Zhao;Fengjun Liu;J. Ling;Jianqiang Lin;Changkai Zhang
中科院分区：
文献类型：
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作者：
Guoying Zhang;Yanxi Zhao;Fengjun Liu;J. Ling;Jianqiang Lin;Changkai Zhang
本研究采用电感耦合等离子体质谱法（ICP-MS）测定了天然和人工栽培的冬虫夏草中锂（Li）、钠（Na）、钾(K)、镓（Ga）、镁（Mg）、锌（Zn）、铁（Fe）、铜（Cu）、锰（Mn）、钒(V)、铬（Cr）、镍（Ni）、钴（Co）、钼（Mo）、硒（Se）、钡（Ba）、锡（Sn）、砷（As）、铅（Pb）、镉（Cd）和汞（Hg）等20种必需、非必需和有毒元素。培养基质、天然基质和天然蠕虫在分析前用微波辅助法消化。采用认证标准物质GBW10015（菠菜）对ICP-MS方法进行了验证。单因素方差分析（ANOVA）结果显示，3种样品中元素含量差异有统计学意义（p<0.05）。除Mg、Zn、Cu外，其他元素含量在天然九州木参基质中含量最高。与线虫相比，野生基质中测定元素的浓度更高。培养基质与天然基质之间元素含量的显著差异可能是由于生长环境不同所致。这一发现突出了ICP-MS元素分析的实用性，并根据其成分增强了九州草作为营养食品的候选价值。
In this study, a total of 20 elements (essential, non-essential and toxic): lithium (Li), sodium (Na), potassium (K), gallium (Ga), magnesium (Mg), zinc (Zn), iron (Fe), copper (Cu), manganese (Mn), vanadium (V), chromium (Cr), nickel (Ni), cobalt (Co), molybdenum (Mo), selenium (Se), barium (Ba), tin (Sn), arsenic (As), lead (Pb) cadmium (Cd) and mercury (Hg) in natural and cultured Cordyceps kyushuensis have been determined by means of inductively coupled plasma mass spectrometry (ICP-MS). Cultured stroma, natural stroma and natural worm were digested by microwave-assisted method before analysis. The proposed ICP-MS method was validated by analyzing a certified reference material (CRM) GBW10015 (spinach). The results of one-way analysis of variance (ANOVA) revealed that the element concentrations in the three kinds of samples were significantly different (p<0.05). Except for Mg, Zn, Cu, the values of other elemental contents were the highest in the stroma of natural C. kyushuensis. In comparison with the worm, the concentrations of determined elements in wild stroma were higher. The remarkable difference of elemental contents between cultured and natural stroma may be caused by distinct growing environment. This finding highlighted the usefulness of ICP-MS elemental analysis and enhanced the value of C. kyushuensis as a candidate for nourishing food based on its composition.