Interaction Between Gastric Carcinoma Cells and Neural Cells Promotes Perineural Invasion by a Pathway Involving VCAM1
Interaction Between Gastric Carcinoma Cells and Neural Cells Promotes Perineural Invasion by a Pathway Involving VCAM1
复制标题
胃癌细胞和神经细胞之间的相互作用通过涉及 VCAM1 的途径促进神经周围侵袭
DOI：
10.1007/s10620-015-3758-x
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发表时间：
2015-11-01
影响因子：
3.1
通讯作者：
Chen, Lin
中科院分区：
文献类型：
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作者：
Xia, Qijun;Bai, Qing-Ran;Chen, Lin
背景神经周围浸润（PNI）是胃癌局部浸润的重要途径之一，预后差。然而，PNI确切的细胞特征和分子机制尚不清楚。目的探讨胃癌细胞与神经细胞之间的相互作用，以及血管细胞粘附分子1（VCAM 1）方法采用体外细胞共培养方法，研究胃癌细胞与神经细胞之间的细胞和分子相互作用。癌症组织样本在体外肿瘤-神经细胞共培养体系中，高水平VCAM 1的胃癌细胞促进神经前体细胞增殖，诱导神经细胞突起生长和分支。反过来，神经细胞增强肿瘤细胞的向神经迁移和移动性。通过VCAM 1阻断抗体抑制VCAM 1的功能可以减弱这些effictions.ConclusionsOur研究表明，VCAM 1是通过介导的神经-肿瘤相互作用，这是一个互惠互利的过程，在肿瘤侵袭显着参与。胃癌PNI的发生可能与神经细胞和肿瘤细胞之间的相互作用有关。抑制VCAM 1的活性可能是胃癌治疗中针对PNI的潜在策略。
BackgroundPerineural invasion (PNI) is one of the important routes for local spread of gastric carcinoma associated with poor prognosis. However, the exact cellular characteristics and molecular mechanisms of PNI are still unclear.AimTo identify the interaction between gastric carcinoma cells and neural cells, and whether vascular cell adhesion molecule-1 (VCAM1) is involved in this process.MethodsWe adopted in vitro cell coculture assays to investigate the cellular and molecular interaction between gastric cancer cells and neural cells.ResultsWe find upregulation of VCAM1 in clinical gastric cancer tissue samples. In in vitro tumor–neural cell coculture system, gastric cancer cells with high level of VCAM1 promote proliferation of neural progenitor cells and induce the process outgrowth and branching of neural cells. Reciprocally, neural cells enhance neurotropic migration and mobility of tumor cells. Repressing VCAM1 function through VCAM1 blocking antibody can attenuate these effects.ConclusionsOur study indicates that VCAM1 is significantly involved in tumor invasion via mediating nerve–tumor interaction, which is a mutually beneficial process. It is possible that interaction between neural cells and tumor cells might contribute to PNI of gastric carcinoma. Inhibiting the activity of VCAM1 could be a potential strategy targeting PNI in gastric carcinoma therapy.