Effect of epidermal growth factor on the fetal development of the tracheobronchial secretory apparatus in rhesus monkey.
Effect of epidermal growth factor on the fetal development of the tracheobronchial secretory apparatus in rhesus monkey.
复制标题
表皮生长因子对恒河猴气管支气管分泌器胎儿发育的影响。
DOI：
10.1165/ajrcmb/4.2.95
复制
发表时间：
1991
影响因子：
6.4
通讯作者：
Plopper,CG
中科院分区：
文献类型：
--
作者：
StGeorge,JA;Read,LC;Cranz,DL;Tarantal,AF;George-Nascimento,C;Plopper,CG
用重组人表皮生长因子（EGF）处理恒河猴胎儿，以确定EGF是否能诱导气管支气管分泌器的成熟。在75%的妊娠期，EGF同时进入羊水和胎儿腹腔，平均剂量为66 I-tg/kg体重，每种途径超过7天的时间。给药期结束时，分娩胎仔，并立即或在维持呼吸支持6小时后实施安乐死。取出肺，固定气管和右肺的一个肺叶并包埋用于光学显微镜检查。用盐水灌洗左肺，收集的液体用于定量释放的分泌产物。分泌产物也在羊水中以及气管上皮的组织切片上原位测量。当EGF处理的猴子的气管进行了检查，上皮细胞被发现是更高的，并含有更大比例的分泌细胞和更小比例的中间细胞比对照组。然而，每毫米基底层的细胞总数或由基底细胞或纤毛细胞组成的上皮细胞群的比例没有显著变化。EGF处理的猴的肺灌洗液和羊水中有更多的分泌产物储存在上皮和粘膜下腺体中，呼吸道分泌物的数量增加。我们得出结论，EGF治疗加速分化气管粘液分泌细胞，分泌颗粒的细胞数量和分泌产物释放到气道腔的量显着增加的基础上。然而，在气管分化活跃的妊娠晚期给予EGF治疗时，不会刺激上皮细胞增殖。各种激素和生长因子加速了胎肺发育（1）。大多数关于肺发育及其影响因素的文献都集中在肺气体交换区的生长和分化的调节上，而不是气管支气管传导气道。尚未证明影响气体交换区发育的物质会同样影响气管支气管树。例如，糖皮质激素、甲状腺激素、环磷酸腺苷和表皮生长因子（EGF）已被证明可刺激气体交换区的生长和成熟（1-3），但这些物质在气管支气管成熟中的作用尚未得到很好的表征。EGF是一种...
Fetal rhesus monkeys were treated with recombinant human epidermal growth factor (EGF) to determine if EGF can induce maturation of the tracheobronchial secretory apparatus. At 75% of gestation, EGF was administered simultaneously into both the amniotic fluid and fetal abdominal cavity at an average dose of 66 I-tg/kg body weight, by each route over a 7-d period. At the end of the treatment period, the fetuses were delivered and either euthanized immediately or after maintenance on ventilatory support for 6 h. The lungs were removed, and the trachea and one lobe of the right lung was fixed and embedded for light microscopy. The left lung was lavaged with saline, and the collected fluid was used to quantify released secretory product. Secretory product was also measured in amniotic fluid as well as in situ on histologic sections of tracheal epithelium. When the tracheas of EGF-treated monkeys were examined, the epithelium was found to be taller and to contain a greater proportion of secretory cells and a smaller proportion of intermediate cells than the control group. However, there was no significant change in the total number of cells per millimeter of basal lamina or in the proportion of the epithelial population made up of basal or ciliated cells. There was more secretory product stored in the epithelium and submucosal glands and increased quantities of respiratory secretions in both lung lavage and amniotic fluid of EGF-treated monkeys. We conclude that EGF treatment accelerates differentiation of tracheal mucous secretory cells, based on a marked increase in both the number of cells with secretory granules and in the amount of secretory product released into the airway lumen. EGF treatment did not, however, stimulate epithelial proliferation when administered in the third trimester, when the trachea is actively differentiating.Fetal lung development is accelerated by an assortment of hormones and growth factors (1). The majority of the literature on lung development and the factors affecting it have focused on the regulation of growth and differentiation of the pulmonary gas exchange area rather than the tracheobronchial conducting airways. It has not been demonstrated that substances shown to affect the development of the gas exchange area would similarly affect the tracheobronchial tree. For example, glucocorticoids, thyroid hormone, cyclic AMP, and epidermal growth factor (EGF) have been shown to stimulate growth and maturation of the gas exchange area (1-3), but the effectsof these substances in tracheobronchial maturation have not been well characterized. EGF is a poly-