Assembly and Mechanical Properties of the Cargo-Free and Cargo Loaded Bacterial Nanocompartment Encapsulin
Assembly and Mechanical Properties of the Cargo-Free and Cargo Loaded Bacterial Nanocompartment Encapsulin
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DOI：
10.1021/acs.biomac.6b00469
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发表时间：
2016-08-01
影响因子：
6.2
通讯作者：
Heck, Albert J. R.
中科院分区：
文献类型：
--
作者：
Snijder, Joost;Kononova, Olga;Heck, Albert J. R.
原核生物大多缺乏真核细胞典型的膜隔室，但相反，它们具有各种基于蛋白质的细胞器。这些包括细菌微区室，如羧基体和病毒样纳米区室的细菌。胶囊具有用于酶包装的适应性机制，这使其成为携带外来蛋白质货物的有吸引力的平台。在这里，我们调查的组装途径和机械性能的货物无和货物装载的nanocaffecting，使用本地质谱，原子力显微镜和多尺度计算分子建模的组合。我们发现，Ekalin二聚体组装成刚性的单酶细菌容器。此外，我们证明，货物封装有机械影响的外壳。病毒蛋白与病毒衣壳的结构相似性反映在它们的机械性质上。凭借这些强大的机械性能，纳米蛋白为纳米技术应用的开发提供了合适的平台。
Prokaryotes mostly lack membranous compartments that are typical of eukaryotic cells, but instead, they have various protein-based organelles. These include bacterial microcompartments like the carboxysome and the virus-like nanocompartment encapsulin. Encapsulins have an adaptable mechanism for enzyme packaging, which makes it an attractive platform to carry a foreign protein cargo. Here we investigate the assembly pathways and mechanical properties of the cargo-free and cargo-loaded nanocompartments, using a combination of native mass spectrometry, atomic force microscopy and multiscale computational molecular modeling. We show that encapsulin dimers assemble into rigid single-enzyme bacterial containers. Moreover, we demonstrate that cargo encapsulation has a mechanical impact on the shell. The structural similarity of encapsulins to virus capsids is reflected in their mechanical properties. With these robust mechanical properties encapsulins provide a suitable platform for the development of nanotechnological applications.