GENERATION OF NEURONS AND ASTROCYTES FROM ISOLATED CELLS OF THE ADULT MAMMALIAN CENTRAL-NERVOUS-SYSTEM
GENERATION OF NEURONS AND ASTROCYTES FROM ISOLATED CELLS OF THE ADULT MAMMALIAN CENTRAL-NERVOUS-SYSTEM
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DOI：
10.1126/science.1553558
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发表时间：
1992-03-27
期刊：
影响因子：
56.9
通讯作者：
WEISS, S
中科院分区：
文献类型：
--
作者：
REYNOLDS, BA;WEISS, S
哺乳动物中枢神经系统的神经发生被认为在出生后不久就结束了。小鼠纹状体在出生后几天后就不再产生新的神经元。在这项研究中，表皮生长因子诱导从成年小鼠大脑纹状体中分离出的细胞在体外增殖。增殖细胞最初表达巢蛋白（一种在神经上皮干细胞中发现的中间丝），随后发展出神经元和星形胶质细胞的形态和抗原特性。新生成的具有神经元形态的细胞对γ-氨基丁酸和P物质（体内成体纹状体的两种神经递质）具有免疫反应性。因此，成年小鼠纹状体细胞具有分裂和分化为神经元和星形胶质细胞的能力。
Neurogenesis in the mammalian central nervous system is believed to end in the period just after birth; in the mouse striatum no new neurons are produced after the first few days after birth. In this study, cells isolated from the striatum of the adult mouse brain were induced to proliferate in vitro by epidermal growth factor. The proliferating cells initially expressed nestin, an intermediate filament found in neuroepithelial stem cells, and subsequently developed the morphology and antigenic properties of neurons and astrocytes. Newly generated cells with neuronal morphology were immunoreactive for gamma-aminobutyric acid and substance P, two neurotransmitters of the adult striatum in vivo. Thus, cells of the adult mouse striatum have the capacity to divide and differentiate into neurons and astrocytes.