Lateral flow test for visual detection of silver (I) based on cytosine-Ag(I)-cytosine interaction in C-rich oligonucleotides.
Lateral flow test for visual detection of silver (I) based on cytosine-Ag(I)-cytosine interaction in C-rich oligonucleotides.
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DOI：
10.1007/s00604-017-2460-4
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发表时间：
2017-11
期刊：
影响因子：
--
通讯作者：
Guo Z
中科院分区：
文献类型：
--
作者：
Guo Z;Zheng Y;Xu H;Zheng B;Qiu W;Guo Z
作者描述了一种用于视觉检测Ag（I）的基于阿托西肽的侧流试验。该测定基于胞嘧啶-Ag（I）-胞嘧啶[C-Ag（I）-C]配位化学以捕获测试区中的金纳米颗粒（AuNP）标签。通过金-硫醇化学将硫醇化的富C寡核苷酸探针固定在AuNP上，并将生物素化的富C寡核苷酸探针固定在测试区上。用富C寡核苷酸标记的AuNP通过C-Ag（I）-C配位被测试区中的Ag（I）离子捕获。所得AuNP的积累在测试区中产生容易可见的红色带。在优化的条件下，该测试能够目视检测1.0 ppb的Ag（I），这比美国环境保护局定义的饮用水最大允许浓度低50倍。因此，该测试是廉价和高度敏感的。应用于自来水和河水中Ag（I）的测定。我们认为，在资源有限的情况下，测试是一个特别宝贵的工具。我们开发了一种快速、灵敏的银离子的视觉检测方法，使用基于三聚氰胺的侧向流动测试条。该方法依赖于胞嘧啶-Ag +-胞嘧啶（C-Ag+-C）配位化学来捕获侧流装置的测试区上的金纳米颗粒。这导致了一种新的和低成本的快速检测银离子的工具。
The authors describe an oligonucleotide-based lateral flow test for visual detection of Ag(I). The assay is based on cytosine-Ag(I)-cytosine [C-Ag(I)-C] coordination chemistry to capture gold nanoparticle (AuNP) tags in the test zone. A thiolated C-rich oligonucleotide probe was immobilized on the AuNPs via gold-thiol chemistry, and a biotinylated C-rich oligonucleotide probe was immobilized on the test zone. The AuNPs labelled with C-rich oligonucleotides are captured by Ag(I) ions in the test zone through the C-Ag(I)-C coordination. The resulting accumulation of AuNPs produces a readily visible red band in the test zone. Under optimized conditions, the test is capable of visually detecting 1.0 ppb of Ag(I) which is 50 times lower than the maximum allowable concentration as defined by the US Environmental Protection Agency for drinking water. Hence, the test is inexpensive and highly sensitive. It was applied to the detection of Ag(I) in spiked samples of tap water and river water. In our perception, the test is a particularly valuable tool in limited resource settings. We have developed a rapid and sensitive approach for visual detection of silver ions by using oligonucleotide-based lateral flow test strip. The approach relies on cytosine–Ag+–cytosine (C–Ag+–C) coordination chemistry to capture gold nanoparticles on the test zone of lateral flow device. This led to a novel and low-cost tool for rapid detection of silver ions.
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