A Zinc(II) Photocage Based on a Decarboxylation Metal Ion Release Mechanism for Investigating Homeostasis and Biological Signaling.
A Zinc(II) Photocage Based on a Decarboxylation Metal Ion Release Mechanism for Investigating Homeostasis and Biological Signaling.
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DOI：
10.1002/anie.201505778
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发表时间：
2015-10-26
期刊：
影响因子：
--
通讯作者：
Burdette SC
中科院分区：
文献类型：
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作者：
Basa PN;Antala S;Dempski RE;Burdette SC
生物学中的金属离子信号传导已经被广泛地研究了邻硝基苄基光笼;然而，低量子产率和其他光学性质对于这些应用并不理想。我们描述的合成和表征的NTAdeCage，在一类新的Zn 2+光笼，利用光驱动的脱羧反应中的金属离子释放机制的第一个成员。使用改性的次氮基三乙酸螯合剂，NTadeCage以亚pm亲和力结合Zn 2+，并且在解除包裹后金属结合亲和力显示出几乎6个数量级的降低。与其他金属离子光笼相比，NTAdeCage和相应的Zn 2+络合物进行有效的光解，量子产率接近30%。NTadeCage介导表达hZIP 4的非洲爪蟾卵母细胞摄取65 Zn 2+的能力证明了这种光笼化策略执行生物测定的可行性。
Metal ion signaling in biology has been studied extensively with ortho-nitrobenzyl photocages; however, the low quantum yields and other optical properties are not ideal for these applications. We describe the synthesis and characterization of NTAdeCage, the first member in a new class of Zn2+ photocages that utilizes a light-driven decarboxylation reaction in the metal ion release mechanism. NTAdeCage binds Zn2+ with sub-pm affinity using a modified nitrilotriacetate chelator and exhibits an almost 6 order of magnitude decrease in metal binding affinity upon uncaging. In contrast to other metal ion photocages, NTAdeCage and the corresponding Zn2+ complex undergo efficient photolysis with quantum yields approaching 30%. The ability of NTAdeCage to mediate the uptake of 65Zn2+ by Xenopus laevis oocytes expressing hZIP4 demonstrates the viability of this photocaging strategy to execute biological assays.