Neutrophils of Scophthalmus maximus produce extracellular traps that capture bacteria and inhibit bacterial infection
Neutrophils of Scophthalmus maximus produce extracellular traps that capture bacteria and inhibit bacterial infection
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大菱鲆的中性粒细胞产生捕获细菌并抑制细菌感染的细胞外陷阱
DOI：
10.1016/j.dci.2015.11.005
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发表时间：
2016-03-01
影响因子：
2.9
通讯作者：
Sun, Li
中科院分区：
文献类型：
--
作者：
Chi, Heng;Sun, Li
中性粒细胞构成先天免疫系统的重要组成部分。最近，已经发现中性粒细胞产生一种复杂的细胞外结构，称为中性粒细胞胞外陷阱（NETs），可以捕获细菌，真菌和寄生虫。在鱼类中，已经报道了一些关于NET生产的研究，然而，鱼类NET的功能尚不清楚。在这项研究中，我们研究了大菱鲆（Scophthalmus maximus）中性粒细胞产生NET的能力，并研究了大菱鲆NET对细菌感染的影响。我们发现，脂多糖处理后，大菱鲆头肾中性粒细胞产生典型的NET结构，含有DNA和组蛋白。细菌处理还诱导NET的产生，其反过来截留细菌细胞并抑制细菌复制。此外，当将NETS捕获的细菌引入大菱鲆时，其组织传播和定殖能力显着减弱。这些结果首次表明，硬骨鱼NET在体外和体内都具有明显的抗菌作用。(C)2015爱思唯尔有限公司版权所有。
Neutrophils constitute an essential part of the innate immune system. Recently, neutrophils have been found to produce a complex extracellular structure called neutrophil extracellular traps (NETs) that capture bacteria, fungi, and parasites. In fish, a few studies on NETs production have been reported, however, the function of fish NETs is unknown. In this study, we examined the ability of turbot (Scophthalmus maximus) neutrophils to produce NETs and investigated the effect of turbot NETs on bacterial infection. We found that upon lipopolysaccharides treatment, turbot head kidney neutrophils produced typical NETs structures that contained DNA and histones. Bacteria treatment also induced production of NETs, which in turn entrapped the bacterial cells and inhibited bacterial replication. Furthermore, when introduced into turbot, NETS-trapped bacteria exhibited significantly weakened ability of tissue dissemination and colonization. These results indicate for the first time that teleost NETs possess apparent antibacterial effect both in vitro and in vivo. (C) 2015 Elsevier Ltd. All rights reserved.