Comparative analysis of the fusion efficiency elicited by the envelope glycoprotein V1-V5 regions derived from human immunodeficiency virus type 1 transmitted perinatally
Comparative analysis of the fusion efficiency elicited by the envelope glycoprotein V1-V5 regions derived from human immunodeficiency virus type 1 transmitted perinatally
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围产期传播的 1 型人类免疫缺陷病毒的包膜糖蛋白 V1-V5 区引起的融合效率的比较分析。
DOI：
10.1099/vir.0.046771-0
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发表时间：
2012-12-01
影响因子：
3.8
通讯作者：
Kong, Xiaohong
中科院分区：
文献类型：
--
作者：
Guo, Hongyan;Abrahamyan, Levon G.;Kong, Xiaohong
了解在围产期传播人类免疫缺陷病毒1型（HIV-1）的病毒优先建立感染的特性是制定有效措施，以防止传播的关键。先前的一项研究表明，慢性感染母婴对（MIP）的新传播病毒（在婴儿中）在生长方面比相应的慢性感染母亲更适合，这是由其包膜（Env）糖蛋白V1-V5区域赋予的。为了研究在围产期传播过程中，传播病毒的较高适应性是否是由它们由V1-V5区指导的较高进入效率所赋予的，使用两种不同的细胞-细胞融合测定法分析了含有来自5个慢性感染的MIP和2个急性感染的MIP的传播和非传播病毒的V1-V5区的Env的融合性。结果表明，在一个慢性感染的MIP中，与相应的母亲相比，婴儿Env V1-V5诱导了更高的融合效率。此外，V4-V5区在区分这对病毒中的传播和非传播病毒方面发挥了重要作用。然而，在其他MIP中，在母体和婴儿变体之间由V1-V5区域介导的融合性中既没有观察到一致的模式也没有观察到显著差异。这项研究表明，在围产期传播过程中，V1-V5区域指导的病毒适应性选择和进入效率之间没有一致和显著的相关性。其他因素，如传播途径和时间也可能涉及。
Understanding the properties of viruses preferentially establishing infection during perinatal transmission of human immunodeficiency virus type 1 (HIV-1) is critical for the development of effective measures to prevent transmission. A previous study demonstrated that the newly transmitted viruses (in infants) of chronically infected mother-infant pairs (MIPs) were fitter in terms of growth, which was imparted by their envelope (Env) glycoprotein V1-V5 regions, than those in the corresponding chronically infected mothers. In order to investigate whether the higher fitness of transmitted viruses was conferred by their higher entry efficiency directed by the V1-V5 regions during perinatal transmission, the fusogenicity of Env containing V1-V5 regions derived from transmitted and non-tranmsmitted viruses of five chronically infected MIPs and two acutely infected MIPs was analysed using two different cell-cell fusion assays. The results showed that, in one chronically infected MIP, a higher fusion efficiency was induced by the infant Env V1-V5 compared with that of the corresponding mother. Moreover, the V4-V5 regions played an important role in discriminating the transmitted and non-transmitted viruses in this pair. However, neither a consistent pattern nor significant differences in fusogenicity mediated by the V1-V5 regions between maternal and infant variants was observed in the other MIPs. This study suggests that there is no consistent and significant correlation between viral fitness selection and entry efficiency directed by the V1-V5 regions during perinatal transmission. Other factors such as the route and timing of transmission may also be involved.