Overexpression of a Miscanthus lutarioriparius NAC gene MlNAC5 confers enhanced drought and cold tolerance in Arabidopsis.
Overexpression of a Miscanthus lutarioriparius NAC gene MlNAC5 confers enhanced drought and cold tolerance in Arabidopsis.
复制标题
芒草 NAC 基因 MlNAC5 的过度表达赋予拟南芥增强的干旱和寒冷耐受性。
DOI：
10.1007/s00299-015-1756-2
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发表时间：
2015
影响因子：
6.2
通讯作者：
Gongke Zhou
中科院分区：
文献类型：
--
作者：
Xuanwen Yang;Xiaoyu Wang;Lu Ji;Zili Yi;Chunxiang Fu;Jingcheng Ran;Ruibo Hu;Gongke Zhou
Key messageMLNAC5functions as a stress-responsive NAC transcriptionfactor gene and enhances drought and cold stress tolerance in transgenic Arabidopsis via the ABA-dependent signaling pathway.AbstractNAC transcription factors (TFs) play crucial roles in plant responses to abiotic stress.Miscanthus lutarioripariusis one ofMiscanthusspecies native to East Asia. It has attracted much attention as a bioenergy crop because of its superior biomass productivity as well as wide adaptability to different environments. However, the functions of stress-related NAC TFs remain to be elucidated inM. lutarioriparius. In this study, a detailed functional characterization ofMlNAC5was carried out.MlNAC5was a member of ATAF subfamily and it showed the highest sequence identity toATAF1. Subcellular localization of MlNAC5-YFP fusion protein in tobacco leaves indicated that MlNAC5 is a nuclear protein. Transactivation assay in yeast cells demonstrated thatMlNAC5functions as a transcription activator and its activation domain is located in the C-terminus. Overexpression ofMlNAC5in Arabidopsis had impacts on plant development including dwarfism, leaf senescence, leaf morphology, and late flowering under normal growth conditions. Furthermore,MlNAC5overexpression lines in Arabidopsis exhibited hypersensitivity to abscisic acid (ABA) and NaCl. Moreover, overexpression ofMlNAC5in Arabidopsis significantly enhanced drought and cold tolerance by transcriptionally regulating some stress-responsive marker genes. Collectively, our results indicated thatMlNAC5functions as an important regulator during the process of plant development and responses to salinity, drought and cold stresses.
