Hepatitis D virus and hepatitis B virus infection in Amerindian communities of the Amazonas state, Colombia.
Hepatitis D virus and hepatitis B virus infection in Amerindian communities of the Amazonas state, Colombia.
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DOI：
10.1186/s12985-015-0402-5
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发表时间：
2015-10-24
期刊：
影响因子：
4.8
通讯作者：
Navas MC
中科院分区：
文献类型：
--
作者：
di Filippo Villa D;Cortes-Mancera F;Payares E;Montes N;de la Hoz F;Arbelaez MP;Correa G;Navas MC
在哥伦比亚，自1985年以来，丁型肝炎病毒（HDV）感染病例已被正式描述，主要发生在Sierra内华达州de Santa玛尔塔（加勒比海北岸）、Uraba（西北部）和亚马逊（东南部）的美洲印第安人人群中。哥伦比亚最近一次正式报告HDV感染临床病例是在2005年。本研究的目的是确定HDV和/或B型肝炎病毒（HBV）感染的无症状的美洲印第安人从亚马逊州，哥伦比亚东南部，并描述在这一人群中的循环病毒基因型。研究人群在亚马逊州的19个美洲印第安人社区招募。对18岁以上的人群进行了B型肝炎表面抗原（HBsAg）快速检测。从快速检测试剂盒中HBsAg阳性的个体获得的血样通过ELISA分析HBsAg、抗HBc、抗HDV IgM/IgG。用PCR方法检测HBV DNA和HDV RNA。通过测序和系统发育分析确定病毒基因型。通过快速检测，共有23/861人的HBsAg检测呈阳性。43.5%（10/23）的美洲印第安人血清学和/或分子标记显示HDV感染。系统发育分析表明，该人群中HBV F1 b亚型和HDV 3亚型存在排他性循环。哥伦比亚亚马孙州印第安人HBV/HDV感染率为43.5%（10/23）。在861名无症状的美洲印第安人中发现了9例病例;研究中还发现了1例有症状的病例（诊断为终末期肝病）。HDV 3和HBV亚型F1 b的传播表明，这些病毒基因型的不断流动是亚马逊河流域美洲印第安人群体相互作用的结果。进一步的研究是必要的，以确认是否HBV亚型F1 b是流行的人群从东南部地区在哥伦比亚。
In Colombia, cases of Hepatitis D virus (HDV) infection have been officially described since 1985 mainly in Amerindian population from Sierra Nevada de Santa Marta (North Caribbean Coast), Uraba (North West), and Amazon (South East). The last official report of a clinical case of HDV infection in Colombia was registered in 2005. The aims of this study were to identify cases of HDV and/or Hepatitis B virus (HBV) infection in asymptomatic Amerindians from Amazonas state, South East Colombia, and to describe the circulating viral genotypes in this population. The study population was recruited in 19 Amerindian communities in the Amazonas state. Individuals over 18 years old were screened by rapid test for Hepatitis B surface Antigen (HBsAg). Blood samples obtained from individuals positives for HBsAg in the rapid-test assay were analyzed for HBsAg, anti-HBc, anti-HDV IgM/IgG by ELISA. The detection of HBV DNA and HDV RNA was performed by PCR amplification. The viral genotype was determined by sequencing and phylogenetic analysis. A total of 23/861 individuals were positive for HBsAg detection by rapid test. Serological and/or molecular markers of HDV infection were demonstrated in 43.5 % (10/23) of samples from Amerindians. The phylogenetic analysis demonstrated the exclusive circulation of HBV subgenotype F1b of and HDV 3 in this population. A high frequency of HBV/HDV infection was found in Amerindian population from Amazonas State, Colombia (43.5 %, 10/23). Nine cases were identified in a population of 861 asymptomatic Amerindian individuals; one symptomatic case (with diagnosis of end-stage hepatic disease) was also identified in the study. The circulation of HDV 3 and HBV subgenotype F1b suggests a constant flow of these viral genotypes as a result of the interaction of the Amerindian populations from Amazon basin. Further studies are necessary to confirm whether HBV subgenotype F1b is the prevalent in the population from South East region in Colombia.