Time-resolved transcriptomic and proteomic profiling of Heyndrickxia coagulans during NaOH-buffered L-lactic acid production.
Time-resolved transcriptomic and proteomic profiling of Heyndrickxia coagulans during NaOH-buffered L-lactic acid production.
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DOI：
10.3389/fmicb.2023.1296692
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发表时间：
2023
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
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作者：
基于氢氧化钠中和的L-乳酸（L-LA）发酵工艺在产品提取过程中表现出环境友好性。然而，与乳酸钙相比，乳酸钠对菌株的显著应激效应阻碍了乳酸发酵。在这项研究中，我们进行了时间分辨的转录组和蛋白质组学分析的Heyndrickxia coagulans DSM 1在NaOH缓冲的L-LA生产。糖酵解基因的表达水平表现出初始增加，随后降低，而三羧酸循环基因在整个发酵过程中表现出初始降低，随后增加。此外，我们还鉴定了由转录因子和ATP结合盒（ABC）转运蛋白组成的基因簇，这些基因簇在整个发酵过程中表现出表达水平的逐渐升高，并在后期观察到显着上调。本研究为深入了解H.在NaOH缓冲的L-LA发酵过程中的凝结物，并提出了潜在的目标代谢工程。
The L-lactic acid (L-LA) fermentation process, based on sodium hydroxide neutralization, demonstrates environmental friendliness during product extraction. However, lactate fermentation is hindered by the pronounced stress effect of sodium lactate on the strain compared with calcium lactate. In this study, we performed time-resolved transcriptomic and proteomic analyses of Heyndrickxia coagulans DSM1 during NaOH-buffered L-LA production. The expression levels of the glycolytic genes demonstrated an initial increase followed by a subsequent decrease, whereas the tricarboxylic acid cycle genes exhibited an initial decrease followed by a subsequent increase throughout the fermentation process. Moreover, we identified clusters of genes consisting of transcription factors and ATP-binding cassette (ABC) transporters that demonstrate a progressive elevation of expression levels throughout the fermentation process, with significant upregulation observed at later stages. This investigation yields valuable insights into the response mechanisms of H. coagulans during NaOH-buffered L-LA fermentation and presents potential targets for metabolic engineering.
影响因子：
4.2
作者：
Tian, Wenzhe;Qin, Jiayang;Lian, Congcong;Yao, Qingshou;Wang, Xiuwen
通讯作者：
Wang, Xiuwen