A monoclonal antibody against the catalytic domain of PTP1B.
A monoclonal antibody against the catalytic domain of PTP1B.
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DOI：
10.1089/hyb.2011.0115
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发表时间：
2012-06
期刊：
影响因子：
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通讯作者：
Fanwei Zeng;Erru Ni;Shengyu Wang;S. Lv;Chang Cao;Haipeng Zhang;Haiyan Lv;Xiaoli Chen;Jianghua Yan;Fanghong Luo
中科院分区：
文献类型：
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作者：
Fanwei Zeng;Erru Ni;Shengyu Wang;S. Lv;Chang Cao;Haipeng Zhang;Haiyan Lv;Xiaoli Chen;Jianghua Yan;Fanghong Luo
蛋白酪氨酸磷酸酶1B（PTP 1B）是蛋白酪氨酸磷酸酶（PTP）家族的成员，在代谢信号传导中起着至关重要的作用，胰岛素和瘦素信号传导已被充分研究。新的证据表明，PTP 1B也与癌症有关。在本研究中，我们报告的建立，通过杂交瘤方法产生的催化结构域的PTP 1B（PTP 1Bc）的单克隆抗体。间接ELISA法测得抗PTP 1Bc单克隆抗体的效价为4.1×10（6）。Western blot和免疫荧光分析表明，该抗体能特异性结合MDA-MB-231和MDA-MB-453细胞中天然的联合收割机PTP 1B。这种针对PTP 1Bc的单克隆抗体有助于增强对PTP 1B相关生理和病理机制的理解，并可能在未来作为糖尿病，肥胖和癌症的治疗剂。
Protein tyrosine phosphatase 1B (PTP1B), a member of the protein tyrosine phosphatase (PTP) family, plays a crucial role in metabolic signaling, with insulin and leptin signaling being well studied. New evidence indicates that PTP1B is also involved in cancer. In the present study, we report on the establishment of a monoclonal antibody specific for catalytic domain of PTP1B (PTP1Bc) generated through the hybridoma method. The monoclonal antibody is measured to have a titer of 4.1×10(6) against PTP1Bc in indirect ELISA. Western blot and immunofluorescent analyses indicated that this antibody can specifically combine native PTP1B in MDA-MB-231 and MDA-MB-453 cells. This monoclonal antibody against PTP1Bc can help enhance the understanding of PTP1B-related physiological and pathological mechanisms and may act as a therapeutic agent for diabetes, obesity, and cancer in the future.