Salivary gland anticoagulants in culicine and anopheline mosquitoes (Diptera:Culicidae).
Salivary gland anticoagulants in culicine and anopheline mosquitoes (Diptera:Culicidae).
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DOI：
10.1093/jmedent/33.4.645
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发表时间：
1996-07
影响因子：
2.1
通讯作者：
K. R. Stark;Anthony A. James
中科院分区：
文献类型：
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作者：
K. R. Stark;Anthony A. James
来自代表2个吸血亚科的9种蚊子的雌性唾液腺提取物延迟人血浆的凝固。所有提取物均显著延迟凝血时间、凝血酶原时间和活化部分凝血活酶时间，表明对常见凝血途径有影响。对凝血因子Xa（FXa）和凝血酶（共同途径的主要组分）进行显色试验，以确定凝血抑制位点。尽管抑制程度存在差异，但所有按蚊都具有凝血酶导向的抗凝剂，库蚊具有FXa导向的抗凝剂。抗凝剂作用部位的差异很可能反映了2个亚家族对脊椎动物止血挑战的长期独立适应。
Female salivary gland extracts from 9 mosquito species representing the 2 hematophagous subfamilies delayed coagulation of human plasma. All extracts significantly delayed recalcification time, prothrombin time, and activated partial thromboplastin time, indicating an effect on the common coagulation pathway. Chromogenic assays to determine the coagulation inhibition site were performed for coagulation factor Xa (FXa) and thrombin, the major components of the common pathway. Although variation existed in the degree of inhibition, all anophelines have thrombin-directed anticoagulants, and culicine mosquitoes have FXa-directed anticoagulants. Differences in the site of action of the anticoagulants most likely reflected the long period of independent adaptation of the 2 subfamilies to the challenges presented by vertebrate hemostasis.