A GENERAL STUDY ON ADSORPTION OF PROTEIN BY TISSUE
A GENERAL STUDY ON ADSORPTION OF PROTEIN BY TISSUE
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DOI：
10.1016/0005-2795(68)90040-8
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发表时间：
1968-01-01
期刊：
影响因子：
--
通讯作者：
KING, CA
中科院分区：
文献类型：
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作者：
KING, CA
131 I标记的血清蛋白与豚鼠组织切片在体外孵育导致形成牢固结合的蛋白质-组织复合物。大量吸附的蛋白质的解吸需要使用强尿素溶液。可用组织表面的蛋白质饱和需要在培养基中使用高浓度的蛋白质（存在大量可用于蛋白质吸附的位点）。以抗体为吸附质时，结合的抗体可与抗原发生反应，表明结合的蛋白质保留在表面位置。碘乙酰胺抑制吸附过程表明，组织巯基是必需的。
Incubation of 131l-labelled serum proteins with guinea pig tissue slices in vitro led to the formation of firmly bound protein-tissue complexes. Desorption of significant amounts of adsorbed protein required the use of strong urea solutions. Saturation of the available tissue surface with protein required the use of a high concentration of protein in the medium (there were a large number of available sites for protein adsorption). When antibody was used as an adsorbate, the bound antibody could react with antigen indicating that the bound protein remained in a superficial position. Iodoacetamide inhibited the adsorption process suggesting that tissue thiol groups are required.