Fluorescence and Cerenkov luminescence imaging
Fluorescence and Cerenkov luminescence imaging
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DOI：
10.1055/s-0037-1616471
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Johannes Schwenck;Kerstin Fuchs;S. Eilenberger;Anna-Maria Rolle;S. C. Vega;Wolfgang M. Thaiss;Florian Maier
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文献类型：
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作者：
Johannes Schwenck;Kerstin Fuchs;S. Eilenberger;Anna-Maria Rolle;S. C. Vega;Wolfgang M. Thaiss;Florian Maier
本文综述了小动物光学成像（OI）在基础研究的不同领域的应用。在过去，OI已被证明是成本和时间有效的，允许实时成像以及高通量分析，并不意味着使用电离辐射（切伦科夫成像应用除外）。因此，这项技术被广泛传播-不仅在地理上，而且在基础研究的不同领域-并以大量出版物为代表。OI最初用于肿瘤学研究，现在正在炎症和传染病以及神经学等其他领域出现。除了基于荧光探针的对比，切伦科夫发光成像（CLI）的可行性最近已在小动物中显示，因此代表了未来应用的新途径。因此，本文将重点介绍OI在炎症、传染病、细胞跟踪以及神经病学中的应用实例，并对CLI进行概述。
Summary This review addresses small animal optical imaging (OI) applications in diverse fields of basic research. In the past, OI has proven to be cost- and time-effective, allows real-time imaging as well as high-throughput analysis and does not imply the usage of ionizing radiation (with the exception of Cerenkov imaging applications). Therefore, this technique is widely spread – not only geographically, but also among very different fields of basic research – and is represented by a large body of publications. Originally used in oncology research, OI is nowadays emerging in further areas like inflammation and infectious disease as well as neurology. Besides fluorescent probe-based contrast, the feasibility of Cerenkov luminescence imaging (CLI) has been recently shown in small animals and thus represents a new route for future applications. Thus, this review will focus on examples for OI applications in inflammation, infectious disease, cell tracking as well as neurology, and provides an overview over CLI.