Differential expression of alternate forms of a Drosophila src protein during embryonic and larval tissue differentiation.
Differential expression of alternate forms of a Drosophila src protein during embryonic and larval tissue differentiation.
复制标题
果蝇 src 蛋白替代形式在胚胎和幼虫组织分化过程中的差异表达。
DOI：
10.1016/0012-1606(90)90198-r
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发表时间：
1990
影响因子：
2.7
通讯作者：
Vincent3rd,WS
中科院分区：
文献类型：
--
作者：
Wadsworth,SC;Muckenthaler,FA;Vincent3rd,WS
Dsrc 28 C基因编码两种主要蛋白质，p66和p55，每种蛋白质都含有酪氨酸激酶结构域。使用单克隆抗体，我们已经完成了详细的调查Dsrc 28 C蛋白在胚胎和幼虫发育过程中的空间表达。许多胚胎组织的分化伴随着Dsrc 28 C表达的诱导。除了发育中的唾液腺表达高水平的p66外，发育中的组织表达Dsrc 28 C的p55形式。值得注意的例子是从神经发生的早期阶段到胚胎发生的剩余阶段表达p55的中枢和外周神经系统的细胞，以及在胚胎阶段10和11的部分期间瞬时表达p55的极细胞。神经系统表达包括中枢神经系统、前感觉器官和外周感觉神经元的细胞体和神经元纤维。在幼虫发育过程中，中枢神经系统内的p55水平仍然很高，但表达模式发生了重大变化。p55逐渐从中枢神经系统的神经元纤维和胚胎细胞体中消失。在第三龄幼虫，出生的腹侧和中脑神经节，但不是在视神经节内的未成熟的神经母细胞，是由短暂的高水平的p55表达的标志。在幼虫中观察到的所有成虫细胞都表达p66蛋白。我们已经注意到的表达模式表明，Dsrc 28 C蛋白的p55形式的表达是神经元细胞分化的早期事件，而p66形式的表达是致力于外胚层细胞分化的细胞的特征。
The Dsrc28C gene encodes two major proteins, p66 and p55, each of which contains a tyrosine kinase domain. Using monoclonal antibodies we have completed a detailed investigation of the spatial expression of Dsrc28C proteins during embryonic and larval development. Differentiation of a number of embryonic tissues is accompanied by the induction of Dsrc28C expression. With the exception of the developing salivary glands which express high levels of p66, developing tissues express the p55 form of Dsrc28C. Notable examples are cells of the central and peripheral nervous systems which express p55 from the early stages of neurogenesis through the remainder of embryogenesis and pole cells which transiently express p55 during portions of embryonic stages 10 and 11. Nervous system expression includes the cell bodies and neuronal fibers of the central nervous system, the anterior sensory organs, and the peripheral sensory neurons. During larval development, p55 levels within the central nervous system remain high but substantial changes in the pattern of expression take place. p55 gradually disappears from the neuronal fibers of the central nervous system and from embryonic cell bodies. During the third larval instar, the birth of immature neuroblasts within the ventral and midbrain ganglia, but not within the optic ganglia, is marked by a transient high level of p55 expression. All imaginal cells that have been observed within the larva express the p66 protein. The patterns of expression that we have noted suggest that expression of the p55 form of Dsrc28C protein is an early event in the differentiation of neuronal cells, while expression of the p66 form is characteristic of cells committed to ectodermal cell differentiation.