The effect of 17 beta-estradiol and progesterone on prostaglandin production by human endometrium maintained in organ culture.
The effect of 17 beta-estradiol and progesterone on prostaglandin production by human endometrium maintained in organ culture.
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17β-雌二醇和黄体酮对器官培养中人子宫内膜产生前列腺素的影响。
DOI：
10.1210/endo-106-5-1599
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发表时间：
1980
期刊：
影响因子：
4.8
通讯作者：
D. Baird
中科院分区：
文献类型：
--
作者：
M. Abel;D. Baird
从18名正常妇女在月经周期的不同阶段收集的子宫内膜保持在器官培养6天。前列腺素F2 α（PGF 2 α）和前列腺素E检测在培养基中晚期增殖（10-14天; n = 13）和晚期分泌（20-26天; n = 5）在不存在外源性类固醇培养的前列腺。在培养的第一个48小时内，分泌期子宫内膜的PGF 2 α输出量显著高于增殖期组织。向培养基中添加17 β-雌二醇，剂量范围为1.0-100.0 ng/ml，可诱导分泌型而非增殖型前列腺素生成因子2 α的显著增加。添加剂量范围为5.0-500.0 ng/ml的孕酮可显著降低增殖性和分泌性组织的PGF 2 α和PGE输出，并阻止17 β-雌二醇刺激的分泌性组织PGF 2 α输出增加。
Endometrium collected from 18 normal women at various stages of the menstrual cycle was maintained in organ culture for periods of 6 days. Both prostaglandin F2 alpha (PGF2 alpha) and PGE were detected in medium from late proliferative (days 10-14; n = 13) and late secretory (days 20-26; n = 5) endometria cultured in the absence of exogenous steroids. During the first 48 h in culture, the output of PGF2 alpha by secretory endometrium was significantly greater than that of proliferative tissue. The addition of 17 beta-estradiol to the culture medium at doses ranging from 1.0-100.0 ng/ml induced a significant increase in the output of PGF2 alpha by secretory but not proliferative endometria. The addition of progesterone at doses ranging from 5.0-500.0 ng/ml significantly reduced the output of PGF2 alpha and PGE by both proliferative and secretory endometria and prevented the 17 beta-estradiol-stimulated increase in PGF2 alpha output by secretory tissue.