A surface-engineered polyetheretherketone biomaterial implant with direct and immunoregulatory antibacterial activity against methicillin-resistant Staphylococcus aureus
A surface-engineered polyetheretherketone biomaterial implant with direct and immunoregulatory antibacterial activity against methicillin-resistant Staphylococcus aureus
复制标题
一种表面工程聚醚醚酮生物材料植入物，对耐甲氧西林金黄色葡萄球菌具有直接和免疫调节抗菌活性
DOI：
10.1016/j.biomaterials.2019.04.008
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发表时间：
2019-07-01
期刊：
影响因子：
14
通讯作者：
Zhang, Xianlong
中科院分区：
文献类型：
--
作者：
Liu, Wei;Li, Jinhua;Zhang, Xianlong
金属离子或纳米颗粒对耐药细菌具有良好的杀菌作用，被认为是开发抗菌生物材料的良好添加剂。然而，金属离子的免疫调节抗菌活性却鲜有报道。本文通过磺化(SPEK)在聚醚酮生物材料表面制备了一种捕获耐甲氧西林金黄色葡萄球菌(MRSA)的多孔微结构，然后利用定制的磁控溅射技术在SPEK表面固定了能够杀死被捕获的耐甲氧西林金黄色葡萄球菌(MRSA)的铜纳米颗粒。体外抗菌实验和免疫学实验表明，含铜的SPEK对MRSA具有较好的杀菌作用，其作用机制为“陷阱杀伤”和“接触杀伤”相结合；同时，含铜SPEK上的巨噬细胞可被激活并极化为促炎表型，并提高对MRSA的吞噬能力。此外，体内植入相关感染模型表明，铜结合的SPEK具有优越的抗菌活性。这些结果表明，铜掺杂的SPEK具有多种抗菌作用，能够同时施加直接抗菌和间接免疫调节抗菌作用，以预防和治疗MRSA感染。相信这项研究将有助于开发集抗菌和免疫调节功能于一体的新型生物材料植入物。
Metal ions or nanoparticles are believed to be promising additives in developing antibacterial biomaterials, owing to possessing favorable bactericidal effects against antibiotic-resistant bacteria. However, the immunomodulatory antibacterial activity of metal ions has seldom been reported. Herein, a porous microstructure designed to trap methicillin-resistant Staphylococcus aureus (MRSA) is fabricated on polyetheretherketone biomaterial surface through sulfonation (SPEEK), following which copper (Cu) nanoparticles, which can kill the trapped MRSA, are immobilized on SPEEK surface using a customized magnetron sputtering technique. In vitro antibacterial and immunological experiments indicate that the Cu-incorporated SPEEK can exert a desirable bactericidal effect against MRSA through the combination of "trap killing" and "contact killing" actions; meanwhile, macrophages cultured on the Cu-incorporated SPEEK can be activated and polarized to a pro-inflammatory phenotype along with improved phagocytic ability on the MRSA. Further in vivo implant-associated infection models evidence the superior antibacterial activity of the Cu-incorporated SPEEK. These results demonstrate multimodal antibacterial actions of the Cu-incorporated SPEEK, which is capable of imposing direct antibacterial and indirect immunomodulatory antibacterial effects simultaneously, in order to prevent and cure MRSA infection. It is believed that this study may shed light on developing novel biomaterial implants that combine antibacterial and immunomodulatory functions.