Accelerated biodegradation of p-tert-butylphenol in the Phragmites australis rhizosphere by phenolic root exudates
Accelerated biodegradation of p-tert-butylphenol in the Phragmites australis rhizosphere by phenolic root exudates
复制标题
酚类根分泌物加速芦苇根际对叔丁基苯酚的生物降解
DOI：
10.1016/j.envexpbot.2019.103891
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发表时间：
2020
影响因子：
5.7
通讯作者：
Zhuobiao Ni
中科院分区：
文献类型：
--
作者：
Dan A;Nichen Zhang;Rongliang Qiu;Charlie Li;Shizhong Wang;Zhuobiao Ni
植物释放的酚类根分泌物（PREs）是降解芳香族化合物的有效途径，但其内在过程和机制尚未完全阐明。在这项研究中，我们选择了四个有代表性的PREs的P。研究了根际PREs对对对叔丁基苯酚（PTBP）生物降解的促进作用及其机理。结果表明，PTBP首先吸附在根表，随后被根际微生物降解。澳大利亚。PHA、PCA和FA的存在有利于PTBP降解菌的富集和PTBP的降解，尤其是PCA的降解。虽然CA也有利于微生物的积累，但它对PTBP的降解没有影响。这可能是因为具有类似PTBP的单羟基结构的PHA、PCA和FA可以促进微生物释放羟化酶催化酚环羟基化，随后是裂解途径，而具有邻位二羟基结构的CA不能作用于该反应。总的来说，我们的数据表明，PREs可以加速特定的芳香族化合物（如PTBP）的生物降解，通过共代谢在植物根际，根据其功能结构。
Phenolic root exudates (PREs) released from plants are potentially effective for the degradation of aromatic compounds, yet the inherent process and mechanism remain insufficiently elucidated. In this study, we selected four representative PREs ofP. australis, namelyp-hydroxybenzoic acid (PHA),p-coumaric acid (PCA), caffeic acid (CA), and ferulic acid (FA), to investigate the accelerated biodegradation ofp-tert-butylphenol (PTBP) by PREs in the rhizosphere and their mechanisms. The results showed that PTBP was initially adsorbed to root surface, and subsequently degraded by rhizosphere microbes ofP. australis. The presence of PHA, PCA, and FA was beneficial for the enrichment of PTBP-degrading bacteria and the decomposition of PTBP, particularly PCA. Although CA was also favorable to microbial accumulation, it had no effect on PTBP degradation. This is possibly because PHA, PCA, and FA with monohydroxy structure similar like PTBP may promote the microbes for releasing hydroxylase to catalyze the phenolic ring hydroxylation followed by a cleavage pathway, whereas CA with ortho-dihydroxy structure cannot act on this reaction. Collectively, our data suggest PREs can accelerate biodegradation of specific aromatic compounds (e.g. PTBP) via co-metabolism in the rhizosphere of plants, according to their functional structures.