Structural determination of picomole amounts of phospholipids via electrospray ionization tandem mass spectrometry
Structural determination of picomole amounts of phospholipids via electrospray ionization tandem mass spectrometry
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DOI：
10.1016/1044-0305(95)00568-4
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发表时间：
1995-12-01
影响因子：
3.2
通讯作者：
Gross, RW
中科院分区：
文献类型：
--
作者：
Han, XL;Gross, RW
利用电喷雾电离的卓越灵敏度，大大提高了合成和生物来源磷脂的串联质谱分析的灵敏度。在此，我们证明：（1）电喷雾电离后的产物离子光谱可以通过使用小于或等于 5 pmol 的磷脂以及小于 2 质量单位的质量选择窗口轻松获得，（2）电喷雾电离方法固有的低能量有利于分析不稳定的分子离子，这些离子在快原子轰击解吸过程中采用的相对较高的能量不易检测到，（3）碰撞诱导的前体离子解离电喷雾常常产生新颖的产物离子模式。总的来说，这些结果强调了电喷雾电离串联质谱法在微量磷脂结构测定中的实用性。
The remarkable sensitivity of electrospray ionization was exploited to achieve great increases in the sensitivity of tandem mass spectrometric analyses of phospholipids derived from both synthetic and biologic sources. Herein, we demonstrate that (1) product-ion spectra after electrospray ionization can be obtained easily by utilizing less than or equal to 5 pmol of phospholipid with a mass-selected window of less than 2 mass units, (2) the low energy inherent in the electrospray ionization method facilitates analysis of labile molecular ions that are not easily detected with the relatively high energy employed during fast-atom bombardment desorption, and (3) collision-induced dissociation of precursor ions generated from electrospray often resulted in novel product-ion patterns. Collectively, these results underscore the utility of electrospray ionization tandem mass spectroscopy for the structural determination of diminutive amounts of phospholipids.