Elucidating Anomalous Protein Diffusion in Living Cells with Fluorescence Correlation Spectroscopy-Facts and Pitfalls
Elucidating Anomalous Protein Diffusion in Living Cells with Fluorescence Correlation Spectroscopy-Facts and Pitfalls
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DOI：
10.1007/s10895-009-0517-4
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发表时间：
2010-01-01
影响因子：
2.7
通讯作者：
Weiss, Matthias
中科院分区：
文献类型：
--
作者：
Malchus, Nina;Weiss, Matthias
在活细胞的细胞内液和细胞膜上经常观察到异常的蛋白质扩散。事实上，从细菌到哺乳动物细胞的大量样本和几种非侵入性测量技术，例如荧光相关光谱，已经揭示了体内蛋白质的均方位移（MSD）通常以异常幂律增加< r（t）（2）>为特征，类似于t（alpha），0.5 < alpha < 0.8。在这里，我们回顾这些结果，特别关注荧光相关光谱，我们报告的异常度α的变化的可能原因。此外，我们强调的异常扩散，可能发挥重要作用的细胞背景下的一般后果。
Anomalous protein diffusion has been frequently observed in intracellular fluids and on membranes of living cells. Indeed, a large variety of specimen, from bacteriae to mammalian cells, and several non-invasive measurement techniques, e.g. fluorescence correlation spectroscopy, have revealed that the mean square displacement (MSD) of proteins in vivo is often characterized by an anomalous power-law increase < r(t)(2)> similar to t(alpha) with 0.5 < alpha < 0.8. Here, we review these results with a particular focus on fluorescence correlation spectroscopy, and we report on possible causes of variations of the anomaly degree alpha. Moreover, we highlight generic consequences of anomalous diffusion that are likely to play an important role in the cellular context.