Dose-dependent hyperbaric oxygen stimulation of human fibroblast proliferation
Dose-dependent hyperbaric oxygen stimulation of human fibroblast proliferation
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DOI：
10.1046/j.1524-475x.1997.50206.x
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发表时间：
1997-01-01
影响因子：
2.9
通讯作者：
Kratz, Gunnar
中科院分区：
文献类型：
--
作者：
Hehenberger, Karin;Brismar, Kerstin;Kratz, Gunnar
糖尿病伤口的特点是伤口愈合过程延长且肉芽组织形成不足。据观察，全身高压氧治疗可以改善这些伤口的愈合。然而，造成这些发现的机制尚未完全阐明。在本研究中，我们研究了高压氧对正常皮肤和非胰岛素依赖型糖尿病患者慢性足溃疡的人类成纤维细胞增殖的体外影响。在氧压为 106 至 300 kPa（795 至 22-50 mm Hg）的高压氧下暴露 1 小时，可增加糖尿病和正常成纤维细胞的增殖。刺激作用是剂量依赖性的，正常细胞和糖尿病细胞的细胞增殖峰值分别在 250 kPa 和 200 kPa 时增加。这些影响并不是由于静水压力本身造成的。这些结果表明高压氧可以刺激糖尿病伤口中的成纤维细胞活性，这一发现可以解释临床上用高压氧治疗的伤口中肉芽组织形成增强的原因。我们还推测，除了增加氧气供应之外，其他机制也可能是我们的发现的原因。
Diabetic wounds are characterized by a prolonged wound healing process with insufficient formation of granulation tissue. Systemic hyperbaric oxygen therapy has been observed to improve the healing of these wounds. However, the mechanism(s) responsible for these findings are not yet fully elucidated. In the present study we have studied the in vitro effects of hyperbaric oxygen on proliferation of human fibroblasts from normal skin and from chronic foot ulcers in non-insulin-dependent diabetics. A 1-hour exposure to hyperbaric oxygen at oxygen pressures between 106 and 300 kPa (795 to 22-50 mm Hg) increased the proliferation in both diabetic and normal fibroblasts. The stimulatory effect was dose-dependent, with a peak increase in cell proliferation at 250 kPa and 200 kPa for normal and diabetic cells, respectively. The effects were not due to hydrostatic pressure per se. These results suggest that hyperbaric oxygen could stimulate fibroblast activity in the diabetic wound, a finding that could explain the enhanced formation of granulation tissue seen clinically in wounds treated with hyperbaric oxygen. We also speculate that mechanisms other than just increased oxygen availability may be responsible for our findings.