Dissociated skin cells regenerate hair follicles in a microwound, "The Punch Assay"
Dissociated skin cells regenerate hair follicles in a microwound, "The Punch Assay"
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分离的皮肤细胞在微伤口中再生毛囊，“打孔试验”。
DOI：
10.1111/exd.13753
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发表时间：
2020-03-01
影响因子：
3.6
通讯作者：
Wu, Xunwei
中科院分区：
文献类型：
--
作者：
Leng, Xue;Wang, Ping;Wu, Xunwei
本研究的目的是开发一种新的，易于执行的毛囊再生系统和检测，可以进一步开发用于临床或美容应用。将分离自新生C57 BL/6小鼠或人胎儿头皮组织的解离的表皮和真皮祖细胞悬浮于（10 μ L）F12培养基中，并移液到免疫缺陷小鼠背部皮肤上直径为1或2 mm的穿孔活检伤口中。在移植后3周，尽管随着小鼠细胞的递送，着色的小鼠毛发可以在注射部位有效地形成，但是在人细胞递送后3个月，在宿主小鼠皮肤上没有毛发形成。在相同的条件下，当人皮肤细胞被递送到在重建的人皮肤（hRSK）上产生的2 mm大小的穿孔上时，人毛囊可以再生，所述重建的人皮肤（hRSK）先前在免疫缺陷小鼠的背部上产生，但是毛发形成的效率低。我们证明小鼠和人的再生毛囊均显示正常的组织学和分化标志物;此外，细胞追踪实验证实再生毛囊由移植细胞形成。与其他目前的毛发重建测定相比，打孔测定相对简单，并且在较小的伤口内产生正常毛囊。我们认为，打孔试验是一种更好的细胞致突变性的体内试验。
The purpose of this study was to develop a new, easily executed hair follicle regeneration system and assay, which could be further developed for clinical or cosmetic applications. Dissociated epidermal and dermal progenitor cells, isolated either from neonatal C57BL/6 mice or human foetal scalp tissues, were suspended in (10 mu L) F12 medium and pipetted into a 1 or 2 mm-diameter punch biopsy wounds on the back skin of immunodeficient mice. At 3 weeks after transplantation, although pigmented mouse hairs could efficiently form at the injection sites with delivery of mouse cells, none hair formed on the host mouse skin at 3 months after delivery of human cells. Under the same conditions, human follicles could be regenerated when the human skin cells were delivered onto a 2 mm size punch created on a reconstituted human skin (hRSK), which previously generated on the back of an immunodeficient mouse, but the efficiency of hair formation was low. We demonstrated that both mouse and human regenerated follicles showed normal histology and differentiation markers; moreover, the cell chasing experiment confirmed that the regenerated hair follicles were formed from transplanted cells. Compared to other current hair reconstituted assays, the Punch Assay is relatively simple and generates normal hair follicles within a smaller wound. We suggest that the punch assay is a better in vivo assay of cell trichogenicity.